| 转基因高粱Cry1Ab蛋白含量的比较研究 |
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| 引用本文： | 张明洲,陈宗伦,方美明,崔海瑞,夏英武.转基因高粱Cry1Ab蛋白含量的比较研究[J].核农学报,2009,23(3):391-394. |
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| 作者姓名： | 张明洲 陈宗伦 方美明 崔海瑞 夏英武 |
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| 作者单位： | 1. 中国计量学院生命科学学院,浙江,杭州,310018
2. 浙江大学原子核农业科学研究所,浙江,杭州,310029 |
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| 基金项目： | 国家自然科学基金，浙江省自然科学基金 |
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| 摘 要： | 通过农杆菌介导法将杀虫晶体蛋白基因cry1Ab导入高粱恢复系115中，共获得13个独立的转基因株系，26株转基因植株，转化率为5.1%；GUS活性、PCR、Southern和Western杂交分析表明，此基因已整合进高粱基因组并得到正确表达。利用ELISA试剂盒测定Cry1Ab蛋白含量，结果表明，不同转基因植株的Cry1Ab蛋白含量有明显差异，最高可达0.850 μg/g，占可溶性蛋白的0.016%，还有一些转基因植株中不能表达；同一转基因植株的不同组织中表达量有明显差异，其顺序为：叶>颖壳>籽粒>根>茎；不同部位的叶片也有差异，其顺序为：中部叶>基部叶>新叶。
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| 关 键 词： | 转基因高粱 Cry1Ab蛋白 cry1Ab基因 农杆菌 |
| 收稿时间： | 2008-10-05 |
Comparison on Cry1Ab Protein Content in Transgenic Sorghum |
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| ZHANG Ming-zhou,CHEN Zong-lun,FANG Mei-ming,CUI Hai-rui,XIA Ying-wu.Comparison on Cry1Ab Protein Content in Transgenic Sorghum[J].Acta Agriculturae Nucleatae Sinica,2009,23(3):391-394. |
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| Authors: | ZHANG Ming-zhou CHEN Zong-lun FANG Mei-ming CUI Hai-rui XIA Ying-wu |
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| Institution: | 1.College of Life Science;China Jiliang University;Hangzhou;Zhejiang 310018;2.Institute of Nuclear Agricultural Sciences;Zhejiang University;Zhejiang 310029 |
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| Abstract: | The insecticidal Bacillus thuringiensis(Bt) cry1Ab gene was introduced into sorghum restoring line 115 efficiently mediated by Agrobacterium tumefaciens.A total of 13 independent transgenic plant lines and 26 transgenic plants were regenerated with the average transformation efficiency of 5.1%.The results of GUS expression,PCR analysis,Southern and Western hybridization showed that the cry1Ab gene had been integrated and expressed exactly in sorghum.The expression level of cry1Ab gene was detected in transg... |
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| Keywords: | transgenic sorghum Cry1Ab protein cry1Ab gene Agrobacterium tumefaciens |
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