抗生素对韭菜根尖培养植株再生的影响

卡那霉素 (Km)和G41 8对韭菜根尖培养植株再生均有抑制作用 ,随浓度提高愈伤组织和芽的分化频率逐渐降低 ,G41 8的抑制作用更加显著 ,当Km取 2 0mg L、G41 8取 1 5mg L时 ,不能诱导成芽。羧苄青霉素 (Crb)和噻孢霉素 (Cef)对韭菜的植株再生也有抑制作用 ,当Crb≥ 3 0 0mg L、Cef≥ 2 0 0mg L时 ,严重影响愈伤组织和芽的分化频率 ,出芽数也迅速减少 ,抑制作用随浓度提高而增强。在所取浓度范围内Timen tin对植株再生的抑制作用较小 ,对愈伤组织和芽的分化频率影响不大 ,但是随着Ti mentin浓度提高 ,出芽数逐渐降低 ,当Timentin =50 0mg L时 ,出芽数降低到对照的49 4%。

EFFECTS OF ANTIBIOTICS ON PLANT REGENERATION in vitro ON CHINESE CHIVE ( Allium tuberosum Rottle)

Kanamycin and G418 showed inhibition effects on plant regeneration in vitro on Chinese chive with the callus and shoot induction rates being reduced as the concentration being higher.The latter showed more several inhibition.When Km=20mg/L or G418=15mg/L,there was no shoots regenerated.Carbenicillin and cefotaxime also had inhibition effects on regeneration when Crb≥300mg/L or Cef≥200mg/L which led to reduction of callus and shoot induction rates,especially of shoot number.The inhibition effects of Timentin were not obvious on callus and shoot differentiation but on shoot numer.There were 23\^8 shoots redifferented in average from calli,which was 49\^4% of control.