| 卫星搭载的甜椒87-2过氧化物同工酶检测和RAPD分子检测初报 |
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| 引用本文： | 刘敏,李金国,王亚林,张赞,薛淮,张纯花,李社荣,郝连元,李广元.卫星搭载的甜椒87-2过氧化物同工酶检测和RAPD分子检测初报[J].核农学报,1999,13(5):291-294. |
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| 作者姓名： | 刘敏 李金国 王亚林 张赞 薛淮 张纯花 李社荣 郝连元 李广元 |
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| 作者单位： | 1. 中国科学院遗传所,北京,100101
2. 中国空间技术研究院,北京,100083 |
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| 摘 要： | 对卫星搭载后育成的甜椒８７ ２ 与地面对照龙椒２ 号进行了过氧化物同工酶检测和 Ｒ Ａ Ｐ Ｄ 分子检测，过氧化物同工酶检测的结果表明：太空椒８７ ２ 的生理活性明显高于地面对照；利用随机引物扩增多态 Ｄ Ｎ Ａ 技术（ Ｒａｎｄｏ ｍｌｙ Ａｍ ｐｌｉｆｉｅｄ Ｐｏｌｙ ｍｏｒｐｈｉｃ Ｄ Ｎ Ａ）检测基因序列多态性，从分子水平上找出卫星搭载的甜椒与地面对照品种间的差异，结果显示：有３８ 个引物得到扩增产物，其中有４ 个产物的扩增物出现差异。
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| 关 键 词： | 卫星搭载 甜椒 同工酶 RAPD |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
PRELIMINARY STUDY ON PEROXIDASE ISOENZYME DETECTION AND RAPD MOLECULAR VERIFICATION FOR SWEET PEPPER 87 2 CARRIED BY A RECOVERABLE SATELLITE |
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| Liu Min,Li Jinguo,Wang Yalin,Zhang Zan,Xue Huai,Zhang Chunhua,Li Sherong,Hao Lianyuan,Li Guangyuan.PRELIMINARY STUDY ON PEROXIDASE ISOENZYME DETECTION AND RAPD MOLECULAR VERIFICATION FOR SWEET PEPPER 87 2 CARRIED BY A RECOVERABLE SATELLITE[J].Acta Agriculturae Nucleatae Sinica,1999,13(5):291-294. |
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| Authors: | Liu Min Li Jinguo Wang Yalin Zhang Zan Xue Huai Zhang Chunhua Li Sherong Hao Lianyuan Li Guangyuan |
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| Abstract: | The detection technology of peroxidase isoenzyme pattern and RAPD(Randomly Amplified Polymorphic DNA) analysis were used to space flown sweet pepper variety of 87 2 carried by a recoverable satellite, and its correspondent ground control Longjiao 2. Determination of pero xidase isoenzyme revealed that the physiological activities of space flown sweet pepper were significantly higher than those of its ground control. To detect the polymorphism of gene sequence and the difference in molecular level, 42 primers were used, among them, 38 primers amplified polymorphic products, and the amplified products from 5 primers of sweet peper 87 2 differed from those of ground control. |
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| Keywords: | RAPD |
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