甘蓝型油菜陕2A细胞质雄性不育的遗传及RAPD标记

本研究以甘蓝型油菜陕 2A细胞质雄性不育系、保持系和F2 分离群体为材料 ,对F2 分离群体的遗传分离情况进行分析 ,结果表明 ,可育与不育株花器存在明显差异 ,符合 3∶1的分离比例 ,因而推断细胞质雄性不育恢复基因受 1对显性基因控制。利用分离群体分组分析法(BSA) ,用 1 0 5个随机引物对细胞质雄性不育恢复基因进行RAPD分析 ,发现有 6个随机引物扩增出多态性差异谱带。引物S62 和S74在可育和不育基因池中扩增出单条特异性谱带所代表的DNA序列 ,很可能与不育系的恢复基因连锁。

STUDY ON INHERITANCE AND RESTORING GENE LINKED RAPD MARKERS OF CYTOPLASMIC MALE STERILITY OF SHAN 2A(Brassica napus L.)

The inheritance of cytoplasmic male sterility (CMS) of Shan 2A(Brassica napus L), were studied in F-2 population. The results of the floral traits suggested that the ratio of the lengths of pistil to stamen could be used to distinguish fertile from sterile plant. The 3∶1 segregation ratio of fertile to sterile in F 2 population indicates that there is only one dominant fertility-restoring gene which contributes to the regenesis of the fertility of Brassica napus L. Bulked segregant analysis (BSA) was used to identify random amplified polymorphic DNA (RAPD) markers linked with the fertility-restoring gene of Cytoplasmic Male Sterility in Shan 2A. Totally 105 random 10-mer primers ware screened for both the DNA samples of fertile and sterile bulks. Six primers produced repeatable and differential polymorphic bands between the paired bulks. The differential main bands produced by primers S 62 and S 74 could be the DNA sequence which links the restoring gene.