平阳霉素对花生体细胞胚胎发生的影响

本文对花生离体培养高频率体细胞胚(体胚)诱导方法,及平阳霉素(PYM)对体胚发生的抑制作用进行了研究,旨在为以PYM作为化学诱变剂进行花生离体诱变提供有效方法.以花生品种花育20号和花育22号成熟种子的胚小叶为外植体,在添加不同浓度2,4-D(0,5,10,15,20mg/L)的MSB5(MS无机盐+B5有机成分)培养...

EFFECT OF PINGYANGMYCIN ON SOMATIC EMBRYOGENESIS IN EMBRYONIC LEAFLET CULTURE OF PEANUT

Efficient somatic embryogenesis and restriction effect of PYM in embryonic leaflet culture of peanut were studied in this paper, which is to provide an effective method for mutation in vitro using PYM as the chemical mutagen. The embryonic leaflets of peanut cv. Huayu No.20 and Huayu No.22 were cultured on MSB5 (MS salts+ B5 vitamins) medium containing 2,4-D (0, 5, 10, 15, 20mg/L) to induce somatic embryos. The results showed that 10mg/L 2,4-D was useful for somatic embryogenesis, the frequency of somatic embryo formation achieved 801% and 92.9% in Huayu No.20 and Huayu No.22, respectively, which was significantly higher than the other treatment, except for 5mg/L and 10mg/L 2,4-D in Huayu No.20 which showed no significant difference. When the explants with somatic embryos were transferred to germination medium added with 4mg/L BAP, the embryos germinated and developed to shoots with a frequency of 85%. With this culture method, the restriction effect of PYM on somatic embryogenesis was studied. The results showed that PYM prohibited somatic embryogenesis remarkably. With the increasing of PYM concentration, the frequencies of somatic embryo formation decreased greatly, while the rate of explants browning was increased. Restriction concentrations of two cultivars were different. According to the explant browning rates and somatic embryo induction rates, it was suggested that 3~4mg/L and 4~5mg/L PYM were the optimum mutagen concentrations for Huayu No.20 and Huayu No.22 respectively, and the optimum period of culture duration was 30d.