电子束辐照对灰葡萄孢菌分生孢子萌发活性及致病力的影响

以灰葡萄孢病菌(Botrytis cinerea)分生孢子为研究对象,采用0.5、1.0、2.0和3.0kGy剂量电子 束辐照灰葡萄孢分生孢子,分别测定5℃和25℃培养条件下辐照对灰葡萄孢分生孢子的萌发活性及致病力的影响.结果表明:25℃和5℃培养条件下,2.0kGy处理分生孢子完全萌发时间比对照分别延迟了5和9d;2...

EFFECT OF ELECTRON BEAM IRRADIATION ON CONIDIAL GERMINATION ACTIVITY AND PATHOGENICITY OF Botrytis cinerea

Conidia of Botrytis cinerea were irradiated by electron beam at 0.5, 1.0, 2.0 and 3.0kGy. The influence of electron beam on the activities of conidial germination and pathogenicity at the temperatures of 5℃ and 25℃ were tested, respectively. The results showed that the electron beam could inhibit germination of conidia and the length of germ tube of Botrytis cinerea, and delay the germination time. It could also decrease the pathogenicity obviously and higher irradiation dose showed stronger effects. Compared with control, the complete germination time of conidia extended to 5 and 9d at the cultivate temperatures of 25℃ and 5℃, after 2kGy of irradiation, and the germination rate was reduced 46.57% and 33.68%, respectively. The inhibition rates of germ tube were 25.12% and 74.29% when cultured 24h. The pathogenicity of Botrytis cinerae to strawberry was reduced significantly. After 2.0kGy irradiation and cultivate at 25℃ for 2d, the disease index was 4.17 and it decreased to 15.28 after cultivation of 5℃ for 15d. Electron beam treatment could inhibit the spore germination and germ tube elongation of Botrytis cinerea significantly, delayed the germination time, and reduced its pathogenicity, the higher the dose, the effect was more obvious.