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| PEG模拟干旱胁迫对石斛DNA表观遗传变化的MSAP分析 | |
| 引用本文： | 樊洪泓,李廷春,李正鹏,林毅,蔡永萍,金青.PEG模拟干旱胁迫对石斛DNA表观遗传变化的MSAP分析[J].核农学报,2011,25(2):363-368. |
| 作者姓名： | 樊洪泓 李廷春 李正鹏 林毅 蔡永萍 金青 |
| 作者单位： | 1. 安徽农业大学生命科学学院,安徽合肥,230036 2. 安徽农业大学生命科学学院,安徽合肥230036;安徽省农业科学研究院,安徽合肥230031 3. 安徽农业大学生命科学学院,安徽合肥230036;安徽科技学院,安徽凤阳233100 |
| 基金项目： | 安徽省自然科学基金青年基金，安徽省高等学校优秀青年人才基金，安徽省教育厅重点自然科学项目，校稳定人才基金，校长青年基金，安徽省中药材产业体系 |
| 摘 要： | 利用甲基化敏感扩增多态性(methylation-sensitive amplification polymorphism,MSAP)技术,研究不同聚乙二醇(PEG-6000)处理浓度下霍山石斛基因组DNA的甲基化水平和变化模式.结果表明:经5%、10%和15%PEG-6000处理的霍山石斛DNA甲基化比率分别为34.... |
| 关 键 词： | 霍山石斛 甲基化敏感扩增多态性 干旱胁迫 DNA甲基化 |
MSAP ANALYSIS OF EPIGENETIC CHANGES IN Dendrobium huoshanense UNDER PEG SIMULATED DROUGHT STRESS |
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| FAN Hong-hong,LI Ting-chun,LI Zheng-peng,LIN Yi,CAI Yong-ping,JIN Qing.MSAP ANALYSIS OF EPIGENETIC CHANGES IN Dendrobium huoshanense UNDER PEG SIMULATED DROUGHT STRESS[J].Acta Agriculturae Nucleatae Sinica,2011,25(2):363-368. | |
| Authors: | FAN Hong-hong LI Ting-chun LI Zheng-peng LIN Yi CAI Yong-ping JIN Qing |
| Institution: | School of Life Sciences, Anhui Agriculture University, Hefei, Anhui230036; Anhui Academy of Agricultural Sciences, Hefei , Anhui230031； Anhui Science and Technology University, Fengyang , Anhui233100 |
| Abstract: | The methylation sensitive amplified polymorphism (MSAP) techniques were used to assess genetic and epigenetic variations of Dendrobium huoshanense under drought stress. Results showed that the level of global DNA methylation decreased with increasing of PEG-6000 concentrations. When the tube seedlings were treated with 5%, 10%, 15% of PEG-6000, the levels of DNA methylation dropped to 34.7%, 32.9% and 30.4%, respectively, compared with that for the control group (37.2%). There was a significantly negative correlation (r=-0。998) between PEG-6000 and DNA methylation. In contrast with the control, under stress of 5%, 10% and 15% PEG-6000, methylation and demethylation of DNA were 12.02%, 12.50%, 15.53% and 8.39%, 8.55%, 8.45%, respectively. The ratios of DNA methylation and demethylation, the polymorphism of DNA methylation increased gradually, accompanied with the enhancement of drought stress. |
| Keywords: | Dendrobium huoshanense MSAP drought stress DNA methylation |
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