丹参遗传多样性的SRAP标记分析

相关序列扩增多态性(SRAP)是一种新发展起来的分子标记。试验通过建立优化的丹参SRAP反应体系,从88对引物组合中筛选得到36对多态性引物组合,对生长在四川中江、陕西商洛、山东临沂、安徽亳州和安徽凤阳5个丹参主产区的6个丹参种群进行了遗传多样性分析。结果表明:36对多态性引物组合共产生782条多态性条带,平均每个引物组合产生21.7条多态性条带,显示了较高的多态性。应用NTSYS软件聚类分析36对引物组合的扩增结果,供试材料分为两大类,Jaccard’s遗传相似系数在0.6774~0.8225之间,说明SRAP技术可有效地应用于丹参的遗传多样性及亲缘关系的研究。

STUDIES ON GENETIC DIVERSITY OF Salvia miltiorrhiza BUNGE WITH A MOLECULAR MARKER SRAP

Sequence-related amplified polymorphism(SRAP) is a newly developed molecular marker technique.In this paper,the genetic diversity of 6 species Salvia miltiorrhiza Bunge which were collected from primary producing area,including Zhongjiang in Sichuan,Shangluo in Henan,Linyi in Shandong,Bozhou and Fengyang in Anhui,were tested by using the optimized SRAP reaction system in the study.The NTSYS software was used to analyze the markers.As a result,36 primer pairs were selected from 88,and amplified 782 polymorphic bands with an average of 21.7 polymorphic bands per primer pair.Cluster analysis using UPGMA method based on the data of SRAP amplified bands by 36 primer pairs showed 6 species of Salvia miltiorrhiza Bunge could be distinguished into two main groups.Jaccard's similarity coefficient ranged from 0.6774～0.8225.The result showed that SRAP molecular marker was efficient to study the genetic diversity of Salvia miltiorrhiza Bunge.