用ISSR分析四川苎麻品种(系)间的遗传关系及雄性不育分子标记的建立

本文从100条ISSR引物中筛选出21条引物,对8个四川苎麻品种(系)间的遗传关系进行了分析。PCR扩增结果表明,21条引物在8份材料中共扩增出86条带,平均每条引物扩增出4.1条,其中多态性位点71个,各引物扩增出的位点数3~8个不等,平均每条引物可以检测到3.4个多态性位点。聚类分析和遗传距离分析结果表明,供试品种川7、川8与其亲本遗传距离较远。3个杂交品种(川7、川8、川9)均表现特有的偏父本遗传现象。此外,本研究用ISSR引物U835筛选到了1个雄性不育分子标记,并将其扩增产物克隆测序,结果表明该序列大小为658bp。根据该序列,此标记被转化成稳定的SCAR标记,可用于苎麻雄性不育分子标记辅助育种。

GENETIC RELATION ANALYSIS OF Boehmeria nivea CULTIVARS OR LINES FROM SICHUAN WITH ISSR AND CONSTRUCTION OF THE MOLECULAR MARKERS LINKED TO MALE STERILE GENG

Inter-simple sequence repeat(ISSR)is an effective molecular marker developed on the basis of microsatellite or simple sequence repeat(SSR).In this study 21 ISSR primers,which was screened from 100 ISSR primers,were used to analyze and identify the relationship of 8 cultivars of Boehmeria nivea.PCR amplification showed that 86 clear and repeatable bands were obtained,in which 71 bands appeared to be polymorphic(82.5%).Genetic distance and cluster analysis indicated that the genetic relationships between hybrids and their parents were not equal,the male parent transfer more genetic loci to their descendants,which indicated that hybrids inherited more genetic information from the male parent.In addition,a specific DNA segment was amplified only in the fertile cultivars using the primer U835,and the ISSR-marker was converted into SCAR marker.Which could be used as a male sterile molecular marker-assisted selection of Boehmeria nivea breeding.