冷诱导基因转录因子CBF1的组成型表达对植物的抗寒性及生长发育的影响

通过农杆菌介导法将玉米泛素启动子(Pubi)调控的CBF1基因以及CaMV35启动子调控的GUS基因一起转化烟草,研究了冷诱导基因的转录因子CBF1(CRT/DRE binding factor 1)对植物抗寒性及生长发育的影响。结果表明:CBF1的组成型表达增强了冷敏感植物的抗寒性;在表型上使植株矮壮,叶片着生密集,叶色深绿,叶厚度增加;促进侧枝生长,延长营养生长期,推迟花期;对植株的结实性无明显影响。植株抗寒能力以及表型变化程度与CBF1的表达强度相关。因此,利用CBF1基因进行植物遗传改良时应根据物种的应用特点采用合适的表达调控策略。

EFFECT OF CONSTITUTED EXPRESSION OF CBF1 TRANSCRIPTIONAL FACTOR ON PLANT COLD TOLERANCE,GROWTH AND DEVELOPMENT

The effects of CBF1 on cold tolerance,growth and development of low-temperature sensitive plant were investigated by delivering Arabidopsis CBF1 gene(cbf1) concatenated with GUS gene(udiA) into tobacco.The expression levels of exotic genes in transformed plant were shown by the GUS activity.The results showed that the overexpression of CBF1 could obviously improve cold tolerance of plant.Compared with non-transformed control plants,the transformed plants had a shorter and stronger stem,more leaves that were bottle-green and thicker,and a longer growth period.CBF1 overexpression also promoted the growth of axillary shoots,but had little effect on the fecundity of plants.The degree of the effects depended on the expression level CBF1.Proper regulation strategy for gene expression should be concerned when using CBF1 to modified plants.