| 莱克多巴胺单克隆抗体的制备与初步鉴定 |
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| 引用本文： | 曲勍,齐孟文.莱克多巴胺单克隆抗体的制备与初步鉴定[J].核农学报,2005,19(5):393-396. |
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| 作者姓名： | 曲勍 齐孟文 |
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| 作者单位： | 中国农业大学核技术农业应用实验室,北京,100094;中国农业大学核技术农业应用实验室,北京,100094 |
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| 摘 要： | 为了对莱克多巴胺进行酶联免疫法测定,经由混合酸酐法合成莱克多巴胺免疫原,免疫BALB/c小鼠,通过杂交瘤单克隆化技术最终得到了1株名为3E1-C9-E10的能稳定分泌抗莱克多巴胺的单克隆抗体的杂交瘤细胞株。经ELISA测定,细胞培养上清的效价为1×105,腹水的效价达1×107以上,与多巴酚丁胺有24%的交叉反应率,与沙丁胺醇、克伦特罗没有交叉反应率。
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| 关 键 词： | 莱克多巴胺 β-肾上腺素激动剂 单克隆抗体 |
| 文章编号： | 1000-8551（2005）05-393-04 |
| 收稿时间： | 2004-11-29 |
| 修稿时间： | 2004年11月29 |
PREPARATION AND IDENTIFICATIONN OF MONOCLONAL ANTIBODIES AGAINST RACTOPAMINE |
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| QU Qing,QI Meng-wen.PREPARATION AND IDENTIFICATIONN OF MONOCLONAL ANTIBODIES AGAINST RACTOPAMINE[J].Acta Agriculturae Nucleatae Sinica,2005,19(5):393-396. |
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| Authors: | QU Qing QI Meng-wen |
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| Abstract: | In order to prepare the monoclonal antibody agaist Ractopamine (RCT),RCT-conjugated antigen was produced by methods of mixed acid anhydride.The spleen cells of BALB/c mice immunized by RCT-BSA were fused with SP2/0 plasmacytoma cells using PEG4000. A hybridoma cell line of 3E_1-C_9-E_(10) was screened for specificity to RCT and cloned by limited dilution method ,which secreted stable monoclonal antibodies against RCT with indirect ELISA titers of 1×10~5in supernatant , 1×10~7in astices. The McAb of 3E_1-C_9-E_(10) generally had 24% cross-reactivity to Dobutamine, and showed little or no cross-reactivity to Salbutamol and Clenbuterol. |
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| Keywords: | Ractopamine β2adrenergic agonist monoclonal antibody |
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