盐地碱蓬SsDREB基因的克隆与表达分析研究

盐地碱蓬（Suaeda salsa L.）是典型的盐碱地指示性植物。本研究以盐地碱蓬为材料,根据其他植物中干旱应答元件结合蛋白保守区的氨基酸序列设计简并引物,通过PCR扩增到一条长180bp的cDNA片段,继而利用RACE技术克隆了一个盐地碱蓬DREB基因全长cDNA,命名为SsDREB。SsDREBcDNA全长为14...

CLONING AND EXPRESSION ANALYSIS OF SsDREB GENE FROM Suaeda salsa L.

Suaeda salsa L. is a typical saline indicative plant. In this paper, a 180bp fragment was amplified from Suaeda salsa L.using its cDNA as the template and the conservative sequences of other reported DREB protein genes as the primers by PCR. A full-length cDNA with completed open reading frame of 364 amino acids was cloned using the strategy of RACE(rapid amplification of cDNA ends).This cDNA was designated as SsDREB,which contained 1493bp with an untranslated region of 34bp at 5 'end and a polyA tail at the 3' end. The deduced primary structure of SsDREB contained a single AP2 domain by NCBI SMART. The predicted AP2 protein of SsDREB shares 97％and 95％ identity with BpDREB, GhDREB2,respectively. The expression patterns of SsDREB gene in different organs by real-time quantitative PCR showed that SsDREB gene expressed strongly in leaf and weakly in stem.The results of different stresses showed that SsDREB gene was induced by high-salt and drought, not by cold and ABA.