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| 烟粉虱内共生菌groEL基因的PCR扩增与测序 | |
| 引用本文： | 谭周进,谢丙炎,肖启明,杨宇红,冯兰香.烟粉虱内共生菌groEL基因的PCR扩增与测序[J].核农学报,2005,19(1):68-71,54. |
| 作者姓名： | 谭周进 谢丙炎 肖启明 杨宇红 冯兰香 |
| 作者单位： | 1. 湖南农业大学生物安全科技学院,湖南,长沙,410128 2. 中国农业科学院蔬菜花卉,研究所,北京,100081 3. 湖南农业大学植物保护学院,湖南,长沙,410128 |
| 基金项目： | 国家重点基础研究发展规划项目 (2 0 0 2CB1114 0 0 )，农业部农作物病虫草害生物防治资源研究与利用重点开放实验室开放基金 (LOBCR 2 0 0 3 0 1) |
| 摘 要： | 烟粉虱内共生菌groEL基因编码一种 63KD的GroEL蛋白 ,属于分子伴侣cpn60家族成员 ,与烟粉虱传播植物病毒病关系密切。通过PCR对长期生活在甘蓝上的烟粉虱体内groEL基因进行了PCR扩增 ,序列测定表明其长度为 1 668bp ,编码 5 5 5个氨基酸 ,与Genebank中烟粉虱内共生菌groEL(AF1 30 4 2 1 )的核苷酸同源性为 99 82 % ,氨基酸同源性为 99 64%。 |
| 关 键 词： | 内共生细菌 烟粉虱 groEL PCR扩增 |
| 文章编号： | 1000-8551(2005)01-068-04 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
PCR AMPLIFYING AND SEQUENCING OF GgroEL GENE FROM ENDOSYMBIONTS IN Bemisia tabaci |
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| TAN Zhou-jin,XIE Bing-yan,XIAO Qi-ming,YANG Yu-hong,FENG Lan-xiang.PCR AMPLIFYING AND SEQUENCING OF GgroEL GENE FROM ENDOSYMBIONTS IN Bemisia tabaci[J].Acta Agriculturae Nucleatae Sinica,2005,19(1):68-71,54. | |
| Authors: | TAN Zhou-jin XIE Bing-yan XIAO Qi-ming YANG Yu-hong FENG Lan-xiang |
| Institution: | TAN Zhou-jin1 XIE Bing-yan 2* XIAO Qi-ming3 YANG Yu-hong2 FENG Lan-xiang2= |
| Abstract: | GroEL,one member of chaperon cpn60 family, a kind of 63KD protein coded by groEL gene from endosymbionts of Bemisia tabaci,plays important role in virus disease transmitted by Bemisia tabaci. The product of groEL gene is a kind of viral binding protein,which from endosymbionts of Bemisia tabaci on cabbage was amplified by PCR and cloned. The completed nucleotide sequence of the gene was determined. and it has 1668bp nucleotides and encodes 555 amino acids. Comparison showed that this gene has 99.82% similarity on nucleotides level and 99.64% similarity on amino acid level to AF130421,one groEL gene from endosymbionts of Bemisia tabaci in Genebank. = |
| Keywords: | endosymbiont Bemisia tabaci groEL PCR amplifying |
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