小麦多子房性状近等基因系的选育及遗传背景的检测

以矮败小麦为杂交材料,以多子房小麦多II为供体亲本、单子房小麦77(2)为轮回亲本,经过连续回交和自交,初步培育成多子房近等基因系(NIL)M04309-1和M04309-3。应用APAGE技术对NIL及其亲本的遗传相似性进行了比较分析,结果表明:(1)育成的多子房近等基因系多子房、单子房性状稳定,农艺性状与77(2)一致;(2)用醇溶蛋白APAGE分析多子房近等基因系的遗传相似性,发现该NIL选系内、系间及选系与轮回亲本间已达到较高的遗传一致性,但含多子房基因的选系低于单子房选系的遗传相似性;(3)用醇溶蛋白检测NIL,未发现其与导致该近等基因系子房性状差异的基因有关。

BREEDING OF NEAR-ISOGENIC LINES OF MULTI-OVARY CHARACTER IN WHEAT AND THEIR GENETIC BACKGROUND EVALUATION

Using dwarfing sterile wheat as a breeding material,duoII as so donor parent,77(2)as recurrent male parent,two near isogenic lines(NIL),M04309-1and M04309-3 were bred.The gene location of the multi-ovary gene through successive backcrossing and crossing was analyzed using acid polyacrylamide gel electrophoresis(APAGE) and the genetic background of NIL was obtained through comparison with that of DuoII and 77(2),respectively.The results were as follows:(1)NIL(M04309-1and M04309-3) was highly genetic stability for wheat multi-ovary;(2)The genetic background variance of NIL was evaluated by the APAGE of gliadin.Preferable genetic consistency was found in NIL、between NIL and between NIL and recurrent parent;(3)Characteristic bands of the multi-ovary gene was detected,which did not show any polymorphism between NIL and their parent.