| 2019—2020山东小麦区试品系55个基因的等位基因分布 |
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| 引用本文： | 李玮,张敏,宋国琦,李玉莲,高洁,程敦公,李豪圣,李根英.2019—2020山东小麦区试品系55个基因的等位基因分布[J].核农学报,2023,37(1):17-26. |
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| 作者姓名： | 李玮 张敏 宋国琦 李玉莲 高洁 程敦公 李豪圣 李根英 |
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| 作者单位： | 1.山东省农业科学院作物研究所/农业农村部黄淮北部小麦生物学与遗传育种重点实验室/小麦玉米国家工程实验室，山东 济南 2501002.青岛农业大学农学院，山东 青岛 266109 |
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| 基金项目： | 山东省农业良种工程(2019LZGC001);山东省重大科技创新工程(2020CXGC010805) |
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| 摘 要： | 为分析2019—2020年山东省小麦区试参试品系携带优异等位基因情况，利用产量、品质、抗性、开花期等55个基因的64个竞争性等位基因特异性聚合酶链式反应（KASP）标记对126份参试品系进行分子标记检测。结果表明，90%以上KASP标记具有较好的分型结果，可高效地进行基因型鉴定。20个基因的优异等位基因频率高于80%，包括Vrn-A1、Vrn-B1、Vrn-D1、Ppd-A1、Ppd-B1、Ppd-D1、Psy-D1、Glu-A3g、Rht-D1、Pinb-D1、TaCwi-A1-1、TaGW2-6B、TaSus1-7B、TaGASR7-A1、1-feh-w3、TaDreb-B1、PRA1、PRR-B1、TaFT3-B1和TaMOT1-D1;26个基因的优异等位基因频率低于30%，包括Vp1-B1、Ppo-D1、TaPds-B1、Zds-A1、TEF-7A、Lr46、Glu-B3g、TaGS5-A1、TaCwi-4A、1B/1R、Rht-B1、Lr68、TaELF3-B1、Pina-D1、Sbwm1、TaPHS1、Pm21、COMT-3B、TaCKX-D1、TaSdr-B1、Pch1、...
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| 关 键 词： | 小麦品系 分子标记 等位基因 区域试验 |
| 收稿时间： | 2022-04-01 |
Allele Distribution of 55 Genes in Wheat Lines Participating in 2019-2020 Shandong Trial |
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| LI Wei,ZHANG Min,SONG Guoqi,LI Yulian,GAO Jie,CHENG Dungong,LI Haosheng,LI Genying.Allele Distribution of 55 Genes in Wheat Lines Participating in 2019-2020 Shandong Trial[J].Acta Agriculturae Nucleatae Sinica,2023,37(1):17-26. |
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| Authors: | LI Wei ZHANG Min SONG Guoqi LI Yulian GAO Jie CHENG Dungong LI Haosheng LI Genying |
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| Institution: | 1.Crop Research Institute，Shandong Academy of Agricultural Sciences/Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and Huai River Valley，Ministry of Agriculture and Rural Affairs/National Engineering Laboratory for Wheat and Maize，Jinan，Shandong 2501002.College of Agronomy，Qingdao Agricultural University，Qingdao，Shandong 266109 |
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| Abstract: | To analyze gene distribution in wheat lines involved in 2019—2020 Shandong regional trial， one hundred and twenty-six wheat lines were tested with 64 Kompetitive Allele Specific polymerase chain reaction （KASP） markers which were specific to 55 genetic loci. The markers are related to yield， quality， resistance， flower time and etc. Over 90% KASP markers give acceptable genotyping， which provides an efficient technology for gene identification. Favorable allele frequencies of 20 genes are above 80%， including Vrn-A1， Vrn-B1， Vrn-D1， Ppd-A1， Ppd-B1， Ppd-D1， Psy-D1， Glu-A3g， Rht-D1， Pinb-D1， TaCwi-A1-1， TaGW2-6B， TaSus1-7B， TaGASR7-A1， 1-feh-w3， TaDreb-B1， PRR-A1， PRR-B1， TaFT3-B1 and TaMOT1-D1. Favorable allele frequencies of 26 genes are below 30%， including Vp1-B1， Ppo-D1， TaPds-B1， Zds-A1， TEF-7A， Lr46， Glu-B3g， TaGS5-A1， TaCwi-4A， 1B/1R， Rht-B1， Lr68， TaELF3-B1， Pina-D1， Sbwm1， TaPHS1， Pm21， COMT-3B， TaCKX-D1， TaSdr-B1， Pch1， Lr34， Yr15， Fhb1， TaMoc-7A and TaGS-D1， notably，favorable alleles of TaMoc-7A， Lr34， Fhb1， Pch1， Yr15， and TaSdr-B1 are not detected. Favorable allele frequencies of 9 genes are between 30% and 80%， including Pinb2-V， TaPod-A1， Lox-B1， Glu-A1， Glu-D1， TaGS2-B1， TaGW2-6A， TaSus1-7A， and Lr14a. Favorable allele frequencies of 55 genes show polarization trend. Among all tested lines， JinongCH03， CG086， and JinongCH01 are the only one possessing favorable alleles of TaCKX-D1， Pm21， and COMT-3B， which could be used as gene donors for kernel weight， powdery mildew resistance， and stem lignin content improvement and breeding. In this study， genotypes of 55 genes in 126 wheat lines are clarified， allelic distribution of important agronomic genes in wheat lines attending the Shandong regional trial was basically clarified， which will serve as the foundation for marker assistant selection breeding. |
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| Keywords: | wheat lines molecular marker allele regional trial |
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