干制羊肉基因组DNA不同提取方法的比较

为了研究干制加工羊肉基因组DNA的最佳提取方法,本试验采用传统酚-氯仿法、磁珠法、改良CTAB法、离心柱法分别提取干制处理后的羊肉基因组DNA,并对4种方法提取的羊肉基因组DNA浓度、纯度、完整性以及提取所需时间、PCR扩增效果等进行比较。结果表明,采用磁珠法提取DNA的效果更好,DNA浓度为118.87 ng·μL^-1,A₂₆₀/A₂₈₀值为1.89,而且此方法具有提取时间短、效率高、污染小等特点。本研究结果为干制加工羊肉基因组DNA的大批量提取和检测提供了参考依据。

Comparative Study on Different Extraction Methods of Genomic DNA From Dried Mutton

To study the optimal extraction method of extracting genomic DNA from dried mutton, conventional phenol-chloroform extraction method, magnetic beads method, modified CTAB method and centrifugal column method were used to extract the genomic DNA from dried mutton, respectively. The concentration, purity, integrity, extraction time and PCR amplification effect of mutton genomic DNA extracted by the four methods were compared. The results showed that the extraction effect of DNA by magnetic bead method was better than other methods with DNA concentration of 118.87 ng·μL^-1 and A₂₆₀/A₂₈₀ value of 1.89. This method also had several advantages such as short extraction time, high efficiency and less pollution. Our study provides scientific guidance for mass extraction and detection of genomic DNA from dried mutton in practical.