三种辐照方式对黑松花粉萌发和花粉管生长的影响

研究了低能氮离子束、紫外线与γ射线辐照对黑松花粉萌发和花粉管生长的影响。结果表明,低能氮离子注入对黑松花粉的损伤效应与γ射线和紫外线照射的不同,主要表现在氮离子束剂量-萌发率效应曲线呈"马鞍型"和氮离子束注入诱发花粉管顶端肿胀,这说明低能离子束的作用机制可能与γ射线和紫外线的不同。认为花粉是研究离子注入生物学效应机制的理想模式系统。

EFFECTS OF THREE IRRADIATION METHODS ON POLLEN GERMINATION AND POLLEN TUBE GROWTH OF Pinus thunbegii

The effect of 30 keV nitrogen ion beams,UV and γ-rays irradiation on pollen germination and pollen tube growth of Pinus thunbergii were investigated.Results showed that the biological effects induced by ion beam implantation were different from those induced by UV and γ-rays irradiation.The dose response curve of ion implantation treatment presented a particular'saddle-like'pattern, and ion implantation also significantly induced pollen tube tip swelling.These results indicated that low energy ion implantation might work in different way compared to UV and γ-rays irradiation.It was suggested that pollen could be served as an excellent model system to study the underlying mechanisms responsible for biological effects of ion beam irradiation at cellular level.