|
|||||
|
|
| 高效液相色谱法测定大豆中卵磷脂的含量 | |
| 引用本文： | 张德权,陈卫涛,张柏林.高效液相色谱法测定大豆中卵磷脂的含量[J].核农学报,2006,20(5):414-416. |
| 作者姓名： | 张德权 陈卫涛 张柏林 |
| 作者单位： | 1. 中国农业科学院农产品加工研究所,北京,100094 2. 中国农业科学院农产品加工研究所,北京,100094;河北农业大学食品科技学院,河北,保定,071001 3. 北京林业大学生物科技学院,北京,100083 |
| 基金项目： | 中国农业科学院杰出人才基金 |
| 摘 要： | 应用高效液相色谱法(HPLC),选用Kromasil SIL柱,以甲醇/异丙醇/正己烷(60/35/5,V/V)为流动相,采用紫外检测器(205nm)对卵磷脂进行了测定。结果表明,大豆卵磷脂的保留时间为26.035min,定量线性范围2.06~30.9μg(r=0.9999),精密度和稳定性实验相对标准偏差均小于5%,加样回收率达97.41%,表明建立的大豆卵磷脂检测方法灵敏度高、稳定性和重现性好。 |
| 关 键 词： | 高效液相色谱(HPLC) 卵磷脂 大豆 |
| 文章编号： | 1000-8551(2006)05-414-03 |
| 收稿时间： | 04 5 2006 12:00AM |
| 修稿时间： | 2006年4月5日 |
DETECTION OF SOYBEAN PHOSPHATIDYLCHOLINES WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY |
|
| ZHANG De-quan,CHEN Wei-tao,ZHANG Bo-lin.DETECTION OF SOYBEAN PHOSPHATIDYLCHOLINES WITH HIGH PERFORMANCE LIQUID CHROMATOGRAPHY[J].Acta Agriculturae Nucleatae Sinica,2006,20(5):414-416. | |
| Authors: | ZHANG De-quan CHEN Wei-tao ZHANG Bo-lin |
| Institution: | 1 Institute of Agro-Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing 100094; 2 College of Food Science and Technology, Hebei Agriculture University, Baoding, Hebei 071001 ; 3 College of Biology Science and Technology, Beijing Forestry University, Beijing 100083 |
| Abstract: | The determination of soybean phosphatidylcholines using high-performance liquid chromatography at 205nm was investigated.The phosphatidylcholines was separated very well by the mobile phase of methanol/2-propanol/n-hexane(60/35/5,V/V) on the Kromasil SIL column.The results showed that: the retention time of soybean phosphatidylcholines is 26.035 min,the linear range is 2.06～30.9 μg(r=0.9999).The relative standard deviation(RSD) of reproducibility and stability experiments was lower than 5% and the average recovery rate of phosphatidylcholines is 97.41%,indicating that the method was a simple and fast method with more accuracy and reproduciblity to detect phosphatidylcholines. |
| Keywords: | high-performance liquid chromatography phosphatidylcholines soybean |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
| 点击此处可从《核农学报》浏览原始摘要信息 | |
| 点击此处可从《核农学报》下载免费的PDF全文 | |