|
|||||
|
|
| 朝天罐的离体培养与植株再生 | |
| 引用本文： | 胡松梅,禹华芳,龚泽修,李萍,蒋道松,胡雄贵.朝天罐的离体培养与植株再生[J].核农学报,2009,23(4):626-630. |
| 作者姓名： | 胡松梅 禹华芳 龚泽修 李萍 蒋道松 胡雄贵 |
| 作者单位： | 1. 湖南娄底职业技术学院,湖南,娄底,41700 2. 湖南农业大学生物科学技术学院,湖南,长沙,410128 3. 湖南畜牧兽医研究所,湖南,长沙,410153 |
| 摘 要： | 以朝天罐（Osbeckia opipara）的茎段为外植体进行离体培养植株再生研究，结果表明：茎段在适宜的培养基上可形成愈伤组织，并分化成再生植株。培养基MS + 6-BA 2 mg/L + NAA 0.2 mg/L + 蔗糖30 mg/L+ 琼脂7 mg/L，既适合于愈伤组织的诱导，也适合于愈伤组织的增殖和分化，诱导率为83.3％；丛生芽增殖的最佳培养基为MS + 6-BA 0.2 mg/L+ NAA 0.1 mg?L-1 + 蔗糖 50 mg/L + 琼脂 7mg/L，增殖系数为 7.2； 试管苗生根的最佳培养基为1/2 MS + NAA 0.5 mg/L + IBA 0.2 mg/L + 6-BA 0.05 mg/L + 蔗糖 20 mg/L + 琼脂 7 mg/L，生根率为100％。 |
| 关 键 词： | 朝天罐 离体培养 愈伤组织 植株再生 |
| 收稿时间： | 2008-12-17 |
TISSUE CULTURE AND PLANT REGENERATION OF Osbeckia opipara |
|
| HU Song-mei,YU Hua-fang,GONG Ze-xiu,LI Ping,JIANG Dao-song,HU Xiong-gui.TISSUE CULTURE AND PLANT REGENERATION OF Osbeckia opipara[J].Acta Agriculturae Nucleatae Sinica,2009,23(4):626-630. | |
| Authors: | HU Song-mei YU Hua-fang GONG Ze-xiu LI Ping JIANG Dao-song HU Xiong-gui |
| Institution: | 1.Hunan Loudi Vocational and Technical College;Loudi;Hunan 417000;2.College Bio-Science and Technology;Hunan Agriculture University;Changsha;Hunan 410128;3.Hunan Institute of Animal and Veterinary Science;Hunan 410131 |
| Abstract: | Using tender stem as explant,tissue culture and plant regeneration of Osbeckia opipara had been studied.The results indicated that the callus could be induced from tender stem,and then differentiated to regeneration plants in the proper medium.The most appropriate medium for the induction and proliferation of callus as well as for the differentiation of adventitious buds was MS+6-BA 2mg/L+NAA 0.2mg/L+sucrose 30mg/L+agar 7mg/L.The induction rate of callus reached 83.3%.In addition,the optimum medium for mult... |
| Keywords: | Osbeckia opipara tissue culture callus plant regeneration |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
| 点击此处可从《核农学报》浏览原始摘要信息 | |
| 点击此处可从《核农学报》下载免费的PDF全文 | |