| 氮离子束注入诱变筛选万隆霉素高产菌株的研究 |
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| 引用本文： | 张晓勇,林壁润,高向阳,沈会芳.氮离子束注入诱变筛选万隆霉素高产菌株的研究[J].核农学报,2008,22(6):766-769,802. |
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| 作者姓名： | 张晓勇 林壁润 高向阳 沈会芳 |
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| 作者单位： | [1]广东省农业科学院植物保护研究所,广东广州510640 [2]华南农业大学生命科学院,广东广州510642 |
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| 基金项目： | 国家自然科学基金(30471161); 中央级公益科研所基本科研业务费专项资金项目; 广东省自然科学基金(81510640001000010,04002077、06025388) |
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| 摘 要： | 为获得万隆霉素高产菌株,用氮离子束注入万隆霉素产生菌进行诱变处理,并用链霉素抗性筛选法和抑菌圈筛选法进行突变株的筛选。结果表明:通过能量为20keV、剂量为90×2.6×1013N+/cm2的氮离子束注入处理万隆霉素产生菌的分生孢子,筛选出万隆霉素高产菌株WN939,其摇瓶发酵产素水平较出发菌株提高了82.10%。经5代传代培养,其产素水平稳定。
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| 关 键 词： | 氮离子束注入 万隆霉素产生菌 诱变 筛选 |
| 收稿时间： | 2009-12-31 |
| 修稿时间： | 2009-12-31 |
STUDY ON THE SCREENING OF HIGH-PRODUCING WANLONGMYCIN STRAIN BY NITROGEN ION BEAM IMPLANTATION TECHNIQUE |
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| ZHANG Xiao-yong,LIN Bi-run,GAO Xiang-yang,SHEN Hui-fang.STUDY ON THE SCREENING OF HIGH-PRODUCING WANLONGMYCIN STRAIN BY NITROGEN ION BEAM IMPLANTATION TECHNIQUE[J].Acta Agriculturae Nucleatae Sinica,2008,22(6):766-769,802. |
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| Authors: | ZHANG Xiao-yong LIN Bi-run GAO Xiang-yang SHEN Hui-fang |
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| Abstract: | In order to obtain a high-yield strain of wanlongmycin,the spores of wanlongmycin producing strain was mutated by 20 keV N~+ ions implantation with dose of 90×2.6 ×10~(13) N~+/cm~2 implantation and streptomycin resistance screening and inhibition zone method were applied to screen the mutants.The results showed a strain of high-yield of wanlongmycin was obtained and named WN939.The yield of WN939 was about 82.10% higher than that of the original strain by flask fermentation and rather stable through successive transfer of cultures. |
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| Keywords: | nitrogen ion beam injection technique wanlongmycin producing strain mutagensis screening |
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