基于EST-SSR标记的MCID法鉴定冬瓜种质资源

种质资源鉴定是研究与利用冬瓜种质资源的基础。为了明确冬瓜种质资源遗传多样性,本研究基于表达序列标签-简单重复序列(EST-SSR)分子标记的人工绘制品种鉴别示意图(MCID)法鉴定40份冬瓜种质资源。利用筛选出的7对引物对冬瓜种质资源进行PCR扩增,应用MCID法构建冬瓜品种鉴定图。结果表明,利用7对引物扩增的特征性谱带能够将冬瓜品种在分子水平上区分开,同时能够构建40份冬瓜种质资源的品种鉴定图。利用Ntsys2.10e软件进行聚类分析,在遗传相似系数0.77处,将冬瓜资源分为3个类群。品种鉴定图能够快速地找到区分品种的引物,比聚类图更加直观。基于EST-SSR标记的MCID法是一种有效的冬瓜资源鉴定方法。本研究对冬瓜资源鉴定具有重要意义。

Identification of Wax Gourd by Using EST-SSR Markers-Based MCID Method

It is a fundamental work to identify wax gourd (Benincasa hispida Cogn) for exploration and collecting wax gourd recourse as breeding well as new variety combination. In this study, a approach (manual cultivar identification diagram, MCID) by using EST-SSR marker was adopted to distinguish 40 wax gourd varieties. Seven polymorphic EST- SSR primers were used to perform PCR amplification, and identification map of wax gourd varieties was constructed by MCID. The results showed that, we were able to clarity the wax gourd different at the molecular level with EST-SSR, and figure out CID map manually. The cluster analysis by using Ntsys2.10e software revealed the genetic relationship among 40 varieties. Three subgroups were assigned with the genetic similarity coefficient of 0.77. CID map is more intuitive than clustering map. According to CID map, primers can be found to distinguish different varieties. In this study, MCID method based on EST-SSR markers was proved to be reliable and practicable for rapid discern of wax gourd variety, which is of great significance to the identification of wax gourd germplasms.