高油酸花生萌发期耐冷性综合评价及种质筛选

为筛选高油酸花生萌发期耐冷鉴定指标,构建高油酸花生萌发期耐冷性综合评价体系,挖掘高油酸花生耐冷种质资源。本研究利用隶属函数法、主成分因子分析法、相关性分析及聚类分析法对56份高油酸花生种质资源进行耐冷性综合评价及耐冷种质筛选。结果表明,11个耐冷鉴定指标按贡献率大小依次为露白萌发因子、发芽时间因子及子叶鲜重因子,发芽指数及萌发耐冷指数可以作为高油酸花生萌发期耐冷性的最优鉴定指标,高油酸花生种质资源萌发期耐冷性强弱受多指标影响。高油酸花生萌发期耐冷性状综合评价D值范围为0.080~0.754,均值为0.497,不同高油酸花生品种萌发期耐冷性存在较大差异。56份高油酸花生种质资源分为5个耐冷级别,筛选到萌发期耐冷较强材料1份、中等耐冷材料20份、耐冷较差材料29份、耐冷差材料5份及冷敏感型材料1份。本试验筛选到的耐冷种质可以作为高油酸花生耐冷育种和耐冷机理的研究材料。本研究为探明高油酸花生种质资源耐冷性提供了理论参考。

Comprehensive Evaluation of Cold Tolerance and Germplasm Screening of High Oleic Acid Peanut at Germination Stage

To screen the cold tolerance identification indexes, construct a new comprehensive evaluation system for cold tolerance and excavate the cold-tolerant germplasm resources in high oleic acid peanut. In this study, the cold tolerance of 56 high oleic acid germplasms was comprehensively evaluated by membership function method, principal component factor analysis, correlation analysis and cluster analysis, and meanwhile the cold-tolerant germplasm resources were selected. The results showed that the 11 identification indexes of cold tolerance were summed up as germination factor, germination time factor and cotyledon fresh weight factor according to the contribution rate. The germination index and cold tolerance index could be considered as the optimal identification indexes for the cold tolerance of high oleic peanut during germination. However, the cold tolerance of high oleic peanut germplasm resources at germination stage was affected by multiple indexes. The D value of comprehensive evaluation on cold tolerance of high oleic acid peanut at germination stage ranged from 0.080 to 0.754, with an average value of 0.497, suggesting that there was great variation in cold tolerance of different high oleic acid peanut varieties at germination stage. Furthermore, 56 high oleic acid peanut germplasm resources were divided into 5 cold tolerance grades at germination stage, including 1 material with stronger cold tolerance, 20 materials with medium cold tolerance, 29 general materials with poor cold tolerance, 5 materials with bad cold tolerance and 1 material with cold sensitivity. These cold-tolerant germplasms can be used as materials for breeding and research on cold tolerance mechanism of high oleic acid peanut. What’s more, this study also provides a theoretical reference for ascertaining the cold tolerance of high oleic acid peanut germplasm resources.