甘蔗抗褐锈病基因定位亲本间多态性SSR标记筛选

为获得更多用于构建甘蔗遗传连锁图谱可用的多态性SSR标记,本研究以6个高抗甘蔗褐锈病品种和6个高感甘蔗褐锈病品种为亲本,根据感病母本×抗病父本选配12个杂交组合,筛选在亲本间条带清晰、多态性明显、重复性较好的引物。结果表明,组合柳城03-1137×德蔗93-88、Mex105×粤糖00-236亲本间的多态性引物数最多,分别占总数的52.38%和47.62%;其中,10对引物(18-mSSCIR34、22-mSSCIR38、25-mSSCIR48、32-mSSCIR67、51-mSSCIR50、57-MSSCIR21、71-SMC1490CL*、73-SMC278CS*、75-SMC31CUQ*、77-SMC336BS*)在组合柳城03-1137×德蔗93-88中多态性最好,7对引物(22-mSSCIR38、25-mSSCIR48、45-mSSESTC04、51-mSSCIR50、67-mSSCIR9*、76-SMC334BS*、80-SMC486CG*)在Mex105×粤糖00-236中多态性最好,利用筛选得到的引物,更有利于构建分子遗传图谱。本研究结果为抗褐锈病新基因定位和开发与其紧密连锁的分子标记研究提供了一定的理论依据。

Screening of Polymorphic SSR Molecular Markers Between Resistant and Susceptible Parents for Localization of Brown Rust Resistance Gene

In order to find more polymorphic SSR markers which available for constructing the sugarcane genetic linkage maps, 6 brown rust highly resistant varieties and 6 brown rust highly susceptible varieties were used as the parents to construct twelve crosses according to the principle of susceptible female × resistance male, then the primers with clear bands, obvious polymorphism and good repeatability were screened. Results showed that the numbers of polymorphic primers between Liucheng 03-1137 and Dezhe 93-88, Mex 105 and Yuetang 00-236 were the most, with 52.38% and 47.62% of the total respectively; among these polymorphic primers, 10 pairs of primers, including 18-mSSCIR34、22-mSSCIR38、25-mSSCIR48、32-mSSCIR67、51-mSSCIR50、57-MSSCIR21、71-SMC1490CL*、73-SMC278CS*、75-SMC31CUQ*、77-SMC336BS*, showed rich polymorphism in the parental group of Liucheng 03-1137 × Dezhe 93-88, and 7 pairs of primers, including 22-mSSCIR38、25-mSSCIR48、45-mSSESTC04、51-mSSCIR50、67-mSSCIR9*、76-SMC334BS*、80-SMC486CG*, showed rich polymorphism in the parental group of Mex 105 × Yuetang 00-236. These polymorphic primers will contribute to constructing a molecular genetic map. The results of this study provided a theoretical basis for the research on the localization of new brown rust resistance gene and developing their molecular genetics linkage markers.