| 秋水仙素诱导甘菊多倍体研究 |
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| 引用本文： | 莫官站,张启翔,潘会堂,孙明.秋水仙素诱导甘菊多倍体研究[J].核农学报,2010,24(3):527-531. |
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| 作者姓名： | 莫官站 张启翔 潘会堂 孙明 |
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| 作者单位： | 1. 北京林业大学园林学院,北京,100083
2. 北京林业大学园林学院,北京,100083;国家花卉工程技术研究中心,北京,100083 |
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| 基金项目： | "十一五"科技支撑项目,北京林业大学新进教师科研启动基金项目,北京市教育委员会学科建设与研究生培养项目 |
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| 摘 要： | 以甘菊种子为试验材料,研究了不同秋水仙素浓度和处理时间对甘菊多倍体诱导的影响。结果表明:500mg/L的秋水仙素水溶液浸渍种子12h,变异率为23%,达到了极显著差异。根尖染色体压片检查表明,四倍体染色体数为2n=4x=36,并且获得了同源四倍体甘菊1株(2n=4x=36)。四倍体植株表现出叶片下表皮气孔保卫细胞变大,叶绿体数变多,叶片变小,花径变大,舌状花数目减少,管状花数目增多。
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| 关 键 词： | 甘菊 秋水仙素 多倍体育种 |
POLYPLOID OF Dendranthema
lavandulifolium INDUCED BY COLCHICINES |
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| MO Guan-zhan,SUN Ming,PAN Hui-tang,ZHANG Qi-xiang.POLYPLOID OF Dendranthema
lavandulifolium INDUCED BY COLCHICINES[J].Acta Agriculturae Nucleatae Sinica,2010,24(3):527-531. |
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| Authors: | MO Guan-zhan SUN Ming PAN Hui-tang ZHANG Qi-xiang |
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| Institution: | 1.College of Landscope Architecture,Beijing Forestry University,Beijing 100083;
2.China National Engineering Research Centre for Floriculture,Beijing 100083 |
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| Abstract: | The effects of colchicines concentration and treatment time duration on the seeds of Dendranthema lavandulifolium were studied. The results showed that method for polyploidy induction was soaking seeds in 500mg/L colchicum for about 12h and the induction ratio was about 23%, which showed significant difference. Only one tetraploidy plant was obtained with the chromosome number of 2n=4x=36. The tetraploid plant exhibited some morphological and cytological variations, including the larger guard cells size of stomata, more chloroplasts in stomata guard cell, smaller leaves, bigger flower diameter, less number of ray flower and more number of tubular flower. |
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| Keywords: | " target="_blank">Dendranthema lavandulifoliumzz')" href="#"> Dendranthema lavandulifolium colchicine polyploidy breeding |
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