叠氮化钠对大豆M_1的生物学诱变效应

用0.04%的化学诱变剂叠氮化钠处理大豆品种黑农37、抗线2号、东农42号、东农92-070和东农44号,M1代田间调查发现处理后的植株成活率降低,有矮化和晚熟现象。室内考种发现:株高、主茎节数、分枝数、单株粒数、单株重和百粒重处理后的平均数和均数标准误的值与对照相比,变异范围均增加。SDS-PAGE分析表明有贮藏蛋白亚基缺失体出现。因此,利用化学诱变剂叠氮化钠处理大豆种子是研究大豆化学诱变效应的有效途径之一。

M_1 MUTAGENIC EFFECT ON SOYBEAN INDUCED BY NaN_3

NaN_3 of concentration 0.04% was used to treat the soybean varieties Heinong 37,Kangxian 2,Dongnong 42,Dongnong 92_070 and Dongnong 44.In the M_1 generation,the survival numbers of soybean plants induced by NaN_3 are fewer than that of the contrast;a few dwarf soybean plants and plants maturated later were found. In the experiment,the average and the standard error of the culm length,node number,tiller number,individual seeds,individual seeds'weight and hundred seeds'weight were different from the contrast,and the protein subunit lack can be identified in the gel of SDS_PAGE,it is concluded that the variation of the traits were enlarged by NaN_3 treatment,which provide an effective method to research the chemically mutagenic effect for soybean.