不同砧木嫁接黄瓜蜡质合成基因表达分析及其miRNA预测

为研究不同砧木嫁接中黄瓜果实蜡质合成相关基因的表达量变化,本试验以3461黄瓜为接穗,黑籽南瓜和白籽南瓜为砧木,研究黑籽南瓜嫁接株、白籽南瓜嫁接株和自根苗不同果实生长时期(开花前2 d、开花当天、开花后4 d、开花后6 d、开花后8 d)中蜡质合成基因(Cs CER1、Cs CER3、Cs CER4、Cs CER6、Cs CER8、Cs CER10)的表达变化,并利用RNAhybrid、psRNATarget及RegRNA软件对调控这6个蜡质基因的microRNA(miRNA)作出预测。结果表明,不同砧木嫁接处理中蜡质基因表达差异主要体现在开花后4 d的果实中,Cs CER1、Cs CER3、Cs CER6、Cs CER8、Cs CER10基因在黑籽南瓜嫁接株中的表达量明显高于白籽南瓜嫁接株和自根苗。miRNA预测结果显示,cme-miR164可能调控Cs CER1、Cs CER3、Cs CER4、Cs CER6和Cs CER10基因,cme-miR168可能调控Cs CER3、Cs CER4、Cs CER6和Cs CER10基因,cme-miR390可能调控Cs CER6、Cs CER8和Cs CER10基因。推测Cs CER1、Cs CER3、Cs CER6、Cs CER8、Cs CER10这5个基因与黑籽南瓜嫁接导致的蜡质含量增加密切相关,cme-miR164、cme-miR168和cme-miR390可能参与调控黄瓜蜡质合成。本研究结果为探究嫁接导致蜡质含量变化的机制提供了新的理论依据。

Expression Analysis and miRNA Prediction of Wax Synthetic Genes in Cucumber Grafted Onto Different Rootstocks

To provide basis for studying the mechanism underlying grafting induced wax content change,the 3461 cucumber was chosen as the scion and grafted onto Cucurbita ficifolia or Cucubita moschata.The expressions of wax synthetic genes (CsCER1,CsCER3,CsCER4,CsCER6,CsCER8 and CsCER10) were analyzed in cucumber/Cucurbita ficifolia grafted strain,cucumber/ Cucubita moschata grafted strain and self-rooted cucumber at different fruit growth periods (2 days before flowering,the flowering day,4 days after flowering,6 days after flowering,and 8 days after flowering),and the miRNAs regulating the 6 waxy genes were also predicted by RNAhybrid,psRNATarget and RegRNA software.The main differences of the waxy genes'expression in cucumbers grafted onto different rootstocks were observed in the fruits at 4 days after flowering.The expressions of CsCER1,CsCER3,CsCER6,CsCER8 and CsCER10 genes were significantly higher in cucumber/ Cucurbita ficifolia grafted strain,compared with cucumber/ Cucubita moschata grafted strain and self-rooted cucumber.MiRNA projections suggest that cme-miR164 may regulate the expression of CsCER1,CsCER3,CsCER4,CsCER6 and CsCER10,cme-miR168 may regulate the expression of CsCER3,CsCER4,CsCER6 and CsCER10,and cme-miR390 may regulate the expression of CsCER6,CsCER8 and CsCER10.CsCER1,CsCER3,CsCER6,CsCER8 and CsCER10 are predicted to be the genes related with increased wax content in cucumber/Cucurbitaficifolia grafted strain.Cme-miR164,cme-miR168 and cme-miR390 may regulate waxy genes,the results could provide theoretical basis for studying the mechanism underlying grafting induced wax content increase.