水稻赖氨酸和总黄酮含量的QTL定位及环境互作分析

为挖掘多环境下稳定存在的水稻赖氨酸和总黄酮含量相关QTL,以粳稻东农425和长白10号及其衍生的180个株系的F_(6:7)重组自交系(RIL)作为供试群体,采用完备区间作图法(ICIM)和基于混合线性模型的复合区间作图法(MCIM),对2014年和2015年水稻的赖氨酸含量和总黄酮含量进行加性QTL定位及环境互作分析。结果检测到10个影响赖氨酸含量的加性效应QTL和12个影响黄酮含量的加性效应QTL,分布在除第9、第10和第12染色体以外的9条染色体上,其中在第5染色体的RM538~RM1271标记区间内连续2年检测到总黄酮含量QTL。检测到6个存在环境互作效应的赖氨酸含量QTL、4个存在环境互作效应的总黄酮含量QTL,互作贡献率为0.15%~6.73%;一对影响总黄酮含量的上位互作效应的QTL,贡献率为0.99%。本研究结果为水稻赖氨酸和总黄酮含量QTL分子标记辅助育种提供了一定的理论依据。

QTL Mapping and Environment Interaction Analysis of Lysine Content and Total Content of Flavonoids in Rice

In order to detect the stable QTL of rice lysine content and total content of flavonoids,a F6∶7 recombinant inbred lines (RIL) containing 180 lines derived from two Japonica rice Dongnong 425 and Changbai 10 were used as experimental materials,quantitative trait Ioci(QTL) analysis of lysine content and total content of flavonoids in rice from 2014 to 2015 were analyzed by inclusive composite interval mapping (ICIM) and mixed composite interval mapping (MCIM).The results showed that,10 QTL controlling lysine content and 12 QTL controlling total content of flavonoids were located across the chromosomes except chr 9,10 and 12.Among them,the total content of flavonoids acid was detected between RM538-RM1271 repatedly in two years.6 QTL controlling lysine content and 4 QTL controlling total content flavonoids were detected having interaction effects with the environment,the contribution of environmental interaction is 0.15％ to 6.73％;and one QTL pairs with epistatic effects contributed 0.99％.The present study could provide the theoretical basis for marker assisted breeding of lysine content and total content of flavonoids in rice.