原生质体EMS诱变选育杏鲍菇高产菌丝多糖菌株

为了促进杏鲍菇的产品研发,利用甲基磺酸乙酯(EMS)诱变处理杏鲍菇PL7菌株原生质体,筛选菌丝多糖含量显著增加的原生质体诱变菌株。结果表明,EMS诱变原生质体的适宜浓度和处理时间分别为0.20%和15 min;从160个原生质体再生菌株中筛选出与亲本菌株拮抗作用明显的22株诱变株;通过菌丝多糖浓度测定,获得菌丝多糖浓度显著增加的7株诱变菌株;通过菌落生长速度测定、RepPCR分析和出菇试验,最终获得高产菌丝多糖的新型菌株D3-12。本研究结果为杏鲍菇种质资源创新和新品种选育提供了新途径。

Selection of Pleurotus eryngii Strains With High Yield of Mycelial Polysaccharide by EMS Protoplast Mutagenesis

In order to promote the product development of Pleurotus eryngii,the protoplast of strain PL7 was treated by ethyl methyl sulfonate (EMS) to select the mutants with significantly increased contents of mycelial polysaccharide.The results showed that the appropriate EMS concentration and treatment time for protoplast mutagenesis were 0.20％ and 15 minutes respectively.Mutants of 22 were selected from 160 regenerating colonies by antagonistic test.After the measure of mycelial polysaccharide concentration,7 mutants exhibiting significant increase of mycelial polysaccharideconcentration were selected.After observing the growth rate of myelium,Rep-PCR analyses and mushroom cultivation,the strain D3-12 with high yield of mycelial polysaccharide was finally selected.The research provided a new pathway for germplasm innovation and novel variety selection of P.eryngii.