低能氮离子束注入水稻的生物学效应研究

利用能量为25KeV的氮离子束为诱变源,剂量分别为2.0×1017、2.5×1017和3.0×1017N+/cm2对5份水稻材料进行处理,从其后代群体中筛选得到变异株,并对其生物学效应进行研究。结果表明,变异株的叶绿素含量均比对照植株高,最高达到148.67%;3种同工酶(POD、SOD、CAT)的酶活力变化趋势不一致,与对照均有明显差异;AFLP分析表明,变异株与阴性对照条带相似率很高,与阳性对照相似率低。

STUDIES ON BIOLOGICAL EFFECTS OF LOW ENERGY N~+ ON ION BEAM IMPLANTATION RICE

Dry seeds of five varieties of rice were implanted by 25KeV low energy N~+ with doses of 2.0×10~(17),2.5×10~(17) and 3.0×10~(17)N~+/cm~2,respectively.Mutant plants were selected from their progenies and the biological effects of the mutant plants were studied.The results showed that chlorophyll content of mutant plants was higher than that of the control,and one of those is as high as 148.67% of chlorophyll content compared with the control.Isoenzyme activities(POD,CAT and SOD) of mutant plants were different from the control plants.And AFLP analysis showed that the similarity between variant plants and feminine control plants was higher than that between variant plants and masculine control plants.