N~+注入诱变芽孢杆菌选育高产抗菌物质菌株

本研究通过N+注入芽孢杆菌选育高产抗菌物质菌株。注入N+的能量为25keV,剂量为50×2.6×1013、80×2.6×1013、100×2.6×1013、120×2.6×1013和150×2.6×1013N+/m2。结果表明最佳的注入剂量为50×2.6×1013N+/m2,选育出了1株高产菌株(Bacillus subtilisfmbJ224),其抗菌物质产量是出发菌株的1.96倍,经传代培养,其高产性能稳定。同时通过对该菌株的发酵特性研究,发现其产抗菌物质的模式为延滞合成型。

THE SCREENING OF Bacillus subtilis STRAIN WITH HIGH-PRODUCED ANTIMICROBIAL SUBSTANCE USING N~+ ION IMPLANTATION

N~+ ion implantation was used to obtain higher-yield antimicrobial substance.Bacillus subtilis fmbJ was mutated by 25keV N~+ ion implantation with the dose of 50×2.6×10~(13),80×2.6×10~(13),100×2.6×10~(13),120×2.6×10~(13) and 150×2.6×10~(13) N~+/m~2.Results showed that the optimal N~+ ion dose was 50×2.6×10~(13)N~+/m~2,and a strain of high-yield antimicrobials was obtained and named as Bacillus subtilis fmbJ224.Its antimicrobial substance yield was increased by 96% than the initial.The fermentation characteristic of the strain was studied,and the mode of producing antimicrobial substance for the selected strain was arrearage synthesis type.