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卫星搭载小麦SP3代高分子麦谷蛋白亚基的SDS-PAGE分析
引用本文:张素娜,吕金印.卫星搭载小麦SP3代高分子麦谷蛋白亚基的SDS-PAGE分析[J].核农学报,2009,23(2):193-196.
作者姓名:张素娜  吕金印
作者单位:1. 西北农林科技大学理学院,陕西,杨陵,712100
2. 西北农林科技大学生命科学学院,陕西,杨陵,712100
基金项目:农业部农业公益性行业科研专项基金,西北农林科技大学校重点科研项目 
摘    要:利用十二烷基磺酸钠-聚丙烯胺凝胶电泳(SDS-PAGE)技术对返回式卫星搭载小麦两个品种SP3代籽粒的高分子麦谷蛋白亚基(HMW-GS)进行了分析,并按照相关评分方法计算了高分子量麦谷蛋白Glu-1位点的品质得分。结果表明,经卫星搭载可产生较高频率的HMW-GS基因变异,陕253和西农1043 两个品种SP3代HMW-GS组成的变异频率分别为27.08%和27.45%。陕253和西农1043 SP3代的小麦品质得分分别为7分和6分,陕253 SP3代变异株为优质小麦。

关 键 词:卫星搭载  小麦  高分子麦谷蛋白  SDS-PAGE
收稿时间:2009-12-31
修稿时间:2009-12-31

SDS-PAGE Analysis of High Molecular Weight Glutenin Subunits in SP3 from Spaceflight Carried Wheat
ZHANG Su-na,LV Jin-yin.SDS-PAGE Analysis of High Molecular Weight Glutenin Subunits in SP3 from Spaceflight Carried Wheat[J].Acta Agriculturae Nucleatae Sinica,2009,23(2):193-196.
Authors:ZHANG Su-na  LV Jin-yin
Institution:1.College of Science;Northwest A&F University;Yangling;Shaanxi 712100;2.College of Life Science;Shaanxi 712100
Abstract:The compositions of high molecular weight glutenin subunits(HMW-GS)of the third generation(SP_3)of two wheat varieties spaceflight carried were analyzed by SDS-PAGE.The quality score of Glu-1 of each site was calculated according to the quality rating system.The results showed that the sace flight carried could result in a higher frequency of HMW-GS gene mutation.The variance frequency of HMW-GS in SP_3 of Shaan253 and Xinong1043 were 27.08% and 27.45%,and the quality score in SP_3 of Shaan253 and Xinong104...
Keywords:SDS-PAGE
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