Promising genetic resources for resistance to wheat streak mosaic virus and the wheat curl mite in wheat-<Emphasis Type="Italic">Thinopyrum</Emphasis> partial amphiploids and their derivatives |
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| Authors: | Hongjie Li Robert L Conner Qin Chen Robert J Graf André Laroche F Ahmad A D Kuzyk |
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| Institution: | (1) Lethbridge Research Centre, Agriculture and Agri-Food Canada, P.O. Box 3000, T1J 4B1 Lethbridge, Alberta, Canada;(2) Morden Research Station, Agriculture and Agri-Food Canada, Unit 100–101, Route 100, R6M 1Y5 Morden, Manitoba, Canada;(3) Botany Department, Brandon University, R1A 6A9 Brandon, MB, Canada;(4) Present address: Department of Plant Pathology, Washington State University, 99164-6430 Pullman, WA, USA |
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| Abstract: | Wheat streak mosaic virus (WSMV), vectored by the wheat curl mite (WCM),Aceria tosichella Keifer, is one of the most destructive viral diseases of wheat found in many wheat producing areas of the world. Host resistance is the most effective method for controlling this disease and its vector. Symptomatological analysis and enzyme-linked immunosorbent assay (ELISA) were used to characterize WSMV-resistance in wheat-alien partial amphiploid lines and their derivatives. The results showed that most of partial amphiploids derived fromThinopyrum ponticum andTh. intermedium were free of systemic symptoms with very low ELISA readings that were similar to that of the non-inoculated Chinese Spring control. While the partial amphiploid lines 693 and PWM706 were identified as new genetic resources of resistance to WSMV. The present study demonstrated that both symptomatological and ELISA methods efficiently assessed WSMV-resistance in the wheat-alien hybrids and systemic symptom incidence and ELISA absorbance readings were highly correlated (r
2 = 0.8658–0.9323) over time following inoculation. The ELISA results also indicated that the virus did not buildup in the plant tissues of these virus-resistant partial amphiploids. Similar results were observed in chromosome translocation and substitution lines that have the geneWsm1 conferring WSMV resistance. However, the lines containing the geneWsm1 and all the partial amphiploid lines, except Agrotana, were susceptible to the WCM. One line derived from a cross of wheat and Agrotana, was effective in controlling the spread of WSMV and was highly resistant to the WCM. Another line and an accession ofTriticum dicoccoides (Koern.) Schweinf. were highly susceptible to WSMV and WCM. Early disease development was delayed in a new hard red winter cultivar McClintock. The partial WSMV-resistance of McClintock was demonstrated by initially low ELISA readings, and a lower percentage of infected plants than other WSMV-susceptible wheat. The use of the identified promising sources of resistance to WSMV and the WCM in wheat breeding is discussed.LRC Contribution No. 387-01061. |
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| Keywords: | Disease resistance Enzyme-linked immunosorbent assay Th ponticum Th intermedium Wheat curl mite Wheat-Thinopyrum partial amphiploid Wheat streak mosaic virus |
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