Genetic Diversity among the Germplasm Resources of the Genus <Emphasis Type="Italic">Houttuynia</Emphasis> Thunb. in China based on RAMP Markers |
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Authors: | Wu?Wei Email author" target="_blank">Zheng?YouliangEmail author Chen?Li Wei?Yuming Yan?Zehong |
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Institution: | (1) Triticeae Research Institute, Sichuan Agricultural University, Dujiangyan, 611830, China;(2) Agronomy College, Sichuan Agricultural University, Yaan, 625014, Sichuan Province, China |
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Abstract: | The genetic diversity of 70 Houttuynia Thunb. accessions from Sichuan, Chongqing, Guizhou and Jiangsu province in China were
tested by using random amplified microsatellite polymorphism (RAMP) markers. All of the 43 primer combinations were found
to amplify polymorphic products. A total of 304 products were amplified. Of which, 97.7 products were polymorphic. 6.9 polymorphic
bands were amplified by each primer combination on average. The genetic similarity (GS) between the accessions within H. emeiensis
and H. cordata were 0.660 and 0.575, respectively. The GS between two species was 0.525. The GS values between H. emeiensis
and the H. cordata cytotype with the chromosome number of 36 was 0.559, higher than that between H. emeiensis and the cytotypes
of H. cordata with other chromosome numbers. Within the species H. cordata, the genetic variation between cultivated and wild
accessions was insignificant. The results of cluster analysis by using UPGMA method showed that all the tested accessions
could be differentiated by RAMP markers, and classified into 11 groups. Many accessions with the same chromosome numbers could
be classified together. The genetic diversity was more plentiful in mountainous and margin areas of the Sichuan Basin than
at the bottom of the Basin and the highlands or hills surrounding it. It was concluded that there existed higher genetic diversity
at the molecular level (RAMP markers) among the germplasm resources of the genus Houttuynia. The genetic relationships and
phylogeny of the germplasm resources of Houttuynia were also discussed. |
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Keywords: | Genetic diversity Houttuynia H cordata H emeiensis Phylogeny RAMP |
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