AFLP fingerprinting of sesame (<Emphasis Type="Italic">Sesamum indicum</Emphasis> L.) cultivars: identification,genetic relationship and comparison of AFLP informativeness parameters |
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Authors: | Hernán Laurentin Petr Karlovsky |
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Institution: | (1) Department of Biologic Sciences, Agronomy Faculty, Universidad Centroccidental Lisandro Alvarado, vía Agua Viva, Cabudare, estado Lara, Venezuela;(2) Department of Crop Sciences, Georg-August University, Grisebachstrasse 6, 37077, Goettingen, Germany |
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Abstract: | Amplified fragments length polymorphism (AFLP) was used to distinguish 20 cultivars of sesame (Sesamum indicum L.) and to elucidate the genetic relationship among these genotypes. The data were also used to estimate the usefulness of
parameters currently used to assess the informativeness of molecular markers. A total of 339 markers were obtained using 8
primer combinations. Of the bands, 91% were polymorphic. Five primer combinations were able to distinguish all 20 cultivars
used. None of the remaining three primer combinations could distinguish all accessions if used alone, but using all three
combinations reduced the probability of a random match to 5 × 10−5. Polymorphic information content (PIC), resolving power (Rp) and marker index (MI) of each primer combination failed to correlate significantly with the number of genotypes resolved.
Jaccard’s similarity coefficients ranged from 0.31 to 0.78. Fifteen cultivars were grouped by four UPGMA-clusters supported
by bootstrapping values larger than 0.70. The grouping pattern was similar to the grouping generated by principal coordinate
analysis. The results demonstrated that AFLP-based fingerprints can be used to identify unequivocally sesame genotypes, which
is needed for cultivar identification and for the assessment of the genetic variability of breeding stocks. We recommend to
use the number of cultivars identified by a primer combination instead of PIC, Rp and MI; and to calculate the maximal, instead of average probability of identical match by chance in the assessment of the
informativeness of a marker for cultivar identification. |
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Keywords: | AFLP DNA fingerprinting Genotype identification Genotyping Sesamum indicum |
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