HPLC-ICP-MS法测定农业废水中有机砷与无机砷的方法研究

建立了应用高效液相色谱(HPLC)和电感耦合等离子质谱(ICP-MS)联用技术测定农业水环境样品中三价砷(AsⅢ)、一甲基砷(MMA)、二甲基砷(DMA)、五价砷(AsⅤ)4种砷形态的分析方法。试验表明,4种砷形态的线性范围宽(1~300μg·L-1),相关系数(r)均大于0.999 0,方法检出限低(0.7~0.9μg·L-1),精密度好,重复测定7次结果的RSD均小于5%。通过计算加标回收率验证方法的准确性,加标回收率为94%~112%。实际样品的测定结果显示,农田废水中砷的主要存在形态为As(Ⅴ),其次为As(Ⅲ)。

Analysis of Organic and Inorganic Arsenic in Waste Water of Agricultural Environment by High Performance Liquid Chromatography and Inductively Coupled Plasma Mass Spectrometry

A high performance liquid chromatography and inductively coupled plasma mass spectrometric method was established for analyz-ing 4 arsenic speciations in water of agricultural environment, including As(Ⅲ), MMA, DMA and As(Ⅴ). The results showed that the method had a wide linear range(1 ~ 300 μg·L-1)andthelinearcoefficients were more than 0.999 0. The detection limits were very low(0.7~0.9 μg·L-1). All of the relative standard deviations(RSD)were less than 5%. The recoveries were between 94% and 112%. The analysis results showed that As(Ⅴ)and As(Ⅲ)were the main arsenic speciations existed in the waste water of agricultural environment.