堆肥中大肠菌群等粪便污染指标菌的残留状况调查 |
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引用本文: | 龚春明,王晓丹,顾卫兵,染谷孝.堆肥中大肠菌群等粪便污染指标菌的残留状况调查[J].农业环境保护,2010(8):1595-1600. |
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作者姓名: | 龚春明 王晓丹 顾卫兵 染谷孝 |
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作者单位: | [1]厦门市环境保护科研所,福建厦门361006 [2]上海市环境科学研究院,上海200233 [3]南通农业职业技术学院,江苏南通226007 [4]日本佐贺大学农学部,日本佐贺840—0027 |
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摘 要: | 从日本九州地区23处堆肥化设施采集了以牛粪、鸡粪、污泥和餐厨垃圾为原料的堆肥试样29个,调查了大肠菌群等粪便污染指标菌的残留状况。结果表明,使用DESO培养基有11个试样被检出大肠菌群,检出率达38%,菌数在102~106 cfu.g-1的范围。使用API 20E鉴定系统对4个试样中的21个分离纯化菌株进行了菌种鉴定,发现有大肠菌群属的Escherichia coli、Escherichiavulneris、Pantoea sp.和Buttiauxella agrestis,以及非大肠菌群属的Serratia marcescens等肠内细菌科细菌。采用血清凝集试验对5株E.coli的病原性检测结果均为阴性。进一步针对6处堆肥设施的堆肥发酵过程中大肠菌群的消长追踪发现,大肠菌群数从原料到成品出现了逐渐减少直至消失、暂时消失、未完全消失、完全未检出4种走势,显示即使堆肥发酵温度在60℃以上,大肠菌群也有可能通过交叉污染等途径残留在堆肥成品中。
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关 键 词: | 堆肥 大肠菌群 大肠菌 沙门氏菌 残留 |
Investigation on Survival of Coliform Bacteria in Various Composts |
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Institution: | GONG Chun-ming,WANG Xiao-dan,GU Wei-bing,SOMEYA Takashi(1.Xiamen Environmental Protection Institute,Xiamen 361006,China;2.Shanghai Academy of Environmental Science,Shanghai 200233,China;3.Nantong Agricultural Vocational Technology College,Nantong 226007,China;4.Faculty of Agriculture,Saga Univerisity,Saga 840-0027,Japan) |
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Abstract: | Occurrence and survival of fecal-contamination indicator(coliform bacteria)in various compost samples collected from 23 com-posting facilities in Kyushu were investigated by using selective media.Coliform bacteria were detected on desoxycholate agar(DESO)from 11(38%)of 29 product samples(15 cow dung manures,4 poultry manures,2 biosolid composts and 8 food waste composts)at a range of 102 ~106 cfu.g-1 dry matter.From positive samples,21 isolates were purified.from which,species of coliform bacteria(E.coli,E.vulneria,Pan-toea sp.and Buttiauxella agrestis)were identified,other bacteria(Serratia marcescens)were also observed,suggesting that careful identifi-cation was necessary to avoid false positive counting.Isolates of E.coli were tested for slide aggregation with a set of antiserums against pathogenic E.coli serotypes and negative reaction was obtained for all the isolates tested.Fate of fecal-contamination indicators was followed during compost production at 6 compost facilities and 4 patterns were observed:(1)decreased and finally disappeared(,2)decreased,then re-growth occurred in products,(3)decreased to certain extent and remained in products(,4)not detected even from raw materials.These re-sults suggested that some coliform bacteria might survive during compost production even temperature reached to as high as 60 ℃. |
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Keywords: | compost coliform bacteria Escherichia coli Salmonella survival |
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