铜对大球盖菇抗氧化系统的影响

为了了解大球盖菇对铜胁迫的耐受和应答,进一步研究铜对大球盖菇的毒害机理及避免大球盖菇铜污染提供参考,通过液体培养试验研究了不同浓度铜（Cu）（0、50、100、200、400μmol.L-1）对大球盖菇可溶性糖、脯氨酸含量影响和过氧化物酶、过氧化氢酶活性影响。结果表明,随着基质Cu2＋浓度增加,菌丝体内的可溶性总糖和脯氨酸含量显著降低,当Cu2＋浓度达到400μmol.L-1时,其含量分别下降至对照组的24.03%和33.91%。过氧化物酶和过氧化氢酶活性呈先增加、后降低趋势,活性最大时的铜离子浓度分别是200μmol.L-1和100μmol.L-1。大球盖菇在铜胁迫下可溶性糖和脯氨酸含量没有像高等植物那样随铜浓度升高而先升高后下降的现象,过氧化物酶和过氧化氢酶活性的变化趋势与植物相同,但活性最高对应的铜浓度比植物偏低,二者说明大球盖菇对铜胁迫敏感,在栽培时应注意环境及基质的铜污染程度不能高于100μmol.L-1。

Effects of Copper（Ⅱ）Stress on Antioxidant System in Stropharia rugoso-annulata

The study will benefit the research for harm theory with Cu2＋ poison to Stropharia rugoso-annulata.The effect of different Cu2＋ concentration（0,50,100,200,400 μmol.L-1）on the content of soluble sugar,proline,the activities of POD and CAT of Stropharia rugoso-annulata in liquid culture medium was researched.The result showed that the total soluble sugar and proline decreased with increasing of Cu2＋ concentration.At 400 μmol.L-1 of Cu2＋,the proline and total soluble sugar greatly decreased,being 24.03% and 33.91% of CK.The activities of POD and CAT increased firstly then dcreased,and reached the peak when Cu2＋ concentration was 200 μmol.L-1 and 400 μmol.L-1 respectively.The content of soluble sugar and proline in Stropharia rugoso-annulata did not increase at first then decrease with increasing of Cu2＋ concentration as did in a plant,but the activities of POD and CAT in Stropharia rugoso-annulata changed similarly to that in a plant,but Cu2＋] was lower than in a plant when the activities of POD and CAT reached the highest.These results implied that Stropharia rugoso-annulata was highly sensitive to Cu2＋.Therefore,Cu2＋ concentration in environment or culture medium could not be higher than 100 μmol.L-1.