蚯蚓对土壤-植物系统中菲、芘降解的强化效应研究

采用盆栽试验方法,研究了蚯蚓（Pheretima sp.）对土壤-植物系统中菲、芘的去除效果的强化作用及对土壤酶活性的影响。结果表明,在试验浓度范围（0~322 mg.kg-1）内,蚯蚓对供试植物苏丹草（Sorghum vulgare L.）生长促进作用显著。接种蚯蚓的土壤中,苏丹草在菲、芘各浓度下总生物量较未接种蚯蚓土壤分别提高14.41%~25.91%、14.39%~23.9%;蚯蚓活动可促进土壤-植物系统中菲、芘的降解,接种蚯蚓处理中菲、芘各浓度下降解率较未接种蚯蚓处理分别高4.20%~9.76%、3.69%~9.38%。土壤酶活性测定结果也显示蚯蚓活动可增加土壤酶的活性。

Enhancing Effects of Earthworms on the Degradation of Phenanthrene and Pyrene in Soil-plant System

The enhancing effects of earthworms（Pheretima sp.） on the degradation of phenanthrene（Phe） and pyrene（Pyr） in a soil-plant system and their influences on soil enzyme activity were studied by pot experiments in a greenhouse.The results showed that incubation of earthworms in the soils significantly promoted the growth of Sudan Grass（Sorghum vulgare L.） at the concentrations range（0~322 mg·kg-1）.The biomass of tested grass increased by 14.41%~25.91% for Phe and by 14.39%~23.9% for Pye when earthworms were inoculated in the soils compared to those without earthworm incubation.The activities of earthworms could also facilitate the degradation of Phe and Pyr in soil-plant system.The degradation rates of Phe and Pyr in soils increased by 4.20%~9.76% and 3.69%~9.38% compared to those of the controls within the tested PAHs concentrations,respectively.Meanwhile,earthworms also showed a possitive effect in enhancing soil enzyme activity.The activity of the urease and polyphenol oxidase in the contaminated soil system with earthworms incubation increased compared to those contaminated soil system without earthworms incubation.The earthworms were found to enhance the activity of the urease by 3.88%~11.59% in the treatment of phenanthrene contaminated soil and 2.15%~6.90% in the treatment of pyrene contaminated soil;The activity of polyphenol oxidase were increased by 1.91%~7.61% and 2.29%~3.41% in the treatment of phenanthrene and pyrene contaminated soils,respectively.The fitting equation could be established of the activity of the urease and polyphenol oxidase with the concentrations of Phe and Pyr.