畜禽粪便堆肥作为功能微生物载体的研究

以堆肥作为3株功能芽胞细菌液体菌剂的载体,通过优化载体含水量、温度和接种浓度等关键影响因子,以不同时间载体中有效活菌数的变化为指标,探讨堆肥代替草炭作为功能微生物载体的可行性和最适条件。研究结果表明,载体C（鸡粪）、P（猪粪）、M（1∶1鸡粪猪粪）和TP（猪粪＋草炭）在72 h内的有效活菌数均显著低于草炭;混合载体TC2（50%草炭＋50%鸡粪）和TM1（25%草炭＋75%1∶1鸡粪猪粪）的有效活菌数随着时间的延长而增加,其中72 h时TC2的有效活菌数达到11.40×109 cfu.g-1,TM1的有效活菌数达到2.64×109 cfu.g-1,均与草炭无显著差异,因此适宜代替草炭作为功能微生物的载体。采用单因素实验,载体TC2和TM1的最优化影响因子为含水量30%、吸附温度30℃、菌液接种浓度为108cfu.mL-1。

Using Animal Manure as Carrier of Functional Microorganism

To investigate the feasibility and best condition of using compost（utilizing chicken manure,pig manure,rice husk and sawdust as composting materials） as the carrier of functional microorganism instead of turf.The experiment used the compost as the carrier of three strains bacillus inoculums（Bacillus amyloliquefaciens,Bacillus megaterium and Bacillus mucilaginosus）,meanwhile optimized the key factors,such as the water content of carrier,temperature and inoculation concentration.Taking changes of living cell quantity on the carrier at different stages as a standard.The result showed that the number of living cell quantity in chicken manure（C）,pig manure（P）,mix chicken and pig manure（M）,and mix turf with pig manure（TP） in 72 h were much less than that of in turf（T）;and the number of living cell quantity in the 50% turf＋50% chicken manure（TC2） and 25% turf＋75% mix chicken and pig manure（TM1） increased as time goes by;the number of living cell quantity in TC2 and TM1 respectively increased to 11.40×109 cfu·g-1 and 2.64×109 cfu·g-1 at the 72th hour,which have no significant difference comparing to that of T.Therefore,TC2 and TM1 could be used as the carrier of functional microorganism to replace T.Base on the single-factor experiment,the key factors that made TC2 and TM1 the best carrier were：30% water content,30 ℃ temperature and 108 cfu·mL-1 inoculation concentration.