A novel allele of the Arabidopsis phytochelatin synthase 1 gene conferring high sensitivity to arsenic and antimony |
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Authors: | Takehiro Kamiya Toru Fujiwara |
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Institution: | 1. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Yayoi , Bunkyo-ku , Tokyo 113-8657 ukamiyat@mail.ecc.u-tokyo.ac.jp;3. Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences , The University of Tokyo , Yayoi , Bunkyo-ku , Tokyo 113-8657;4. Core Research for Evolutional Science and Technology, Japan Science and Technology Agency , Chuo-ku , Tokyo 103-0027 , Japan |
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Abstract: | To identify genes involved in arsenite As(III)] tolerance, we screened for As(III)-sensitive mutants using ethyl methanesulfonate (EMS)-mutagenized seeds of Arabidopsis thaliana. One mutant with high sensitivity to As(III) was isolated from the screening. It had a mutation in Glu52 of phytochelatin synthase 1 (AtPCS1), and introduction of the wild-type AtPCS1 gene rescued the phenotype, confirming that the mutant represented a new allele of atpcs1 (termed as cad1-7). The expression of AtPCS1 from cad1-7 and cad1-3, a second allele of atpcs1, complemented As-sensitive yeast, but not plants. Our study suggests that AtPCS1 activity is differentially regulated between plants and yeast. |
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Keywords: | Arabidopsis thaliana antimony arsenic phytochelatin |
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