多菌灵降解菌djl-6和啶虫脒降解菌D-2固体菌剂的研发

以蚯蚓粪肥、猪粪肥、鸡粪肥、秸秆肥、米糠、泥炭以及花生壳粉作为载体,以多菌灵降解菌Rhodococcus qingshengii djl-6和啶虫脒降解菌Pigmentiphaga sp. D-2为材料,开展了固体菌剂的研发。接种菌株djl-6及D-2后,各载体均能够促进供试菌株的生长。以蚯蚓粪肥作为载体时,菌株djl-6、D-2有效菌数释放率最高,分别为100.40%和82.03%。在保存120 d时,除花生壳粉外,其余各载体中的活菌数均高于10~7 cfu/g;以蚯蚓粪为载体的djl-6菌剂活菌数达到了7.00×10~8 cfu/g,以猪粪为载体的D-2菌剂活菌数达到了4.29×10~8 cfu/g。固体菌剂保存30 d后进行土壤农药残留降解试验,以蚯蚓粪为载体的djl-6菌剂对5 mg/kg的多菌灵降解率为94.30%,以蚯蚓粪为载体的D-2菌剂对10 mg/kg的啶虫脒降解率为81.87%。研究结果表明,以蚯蚓粪为载体的固体农残降解菌剂活菌数高、保存期长,具有较好的应用前景。

Study of Solid Bacterial Agents for Carbendazim-degrading Strain djl-6 and Acetamiprid-degrading Strain D-2

In this study, earthworm manure, pig manure, chicken manure, straw manure, rice bran, peat, and peanut shell powder were used as carriers. The carbendazim-degrading bacteria Rhodococcus qingshengii djl-6 and the acetamiprid-degrading bacteria Pigmentiphaga sp. D-2 were used as materials. After being inoculated the strains djl-6 and D-2, all carriers could promote the growth of the tested strains. When earthworm manure was used as the carrier, the effective release rates of strains djl-6 and D-2 were the highest, reaching 100.40% and 82.03% respectively. When stored for 120 d, the number of viable bacteria in each carrier except peanut shell powder was higher than 10⁷ cfu/g. The live bacterial count of djl-6 bacterial agent using earthworm manure as a carrier reached 7.00×10⁸ cfu/g, and the live bacterial count of D-2 bacterial agent using pig manure as a carrier reached 4.29×10⁸ cfu/g. After the solid bacterial agent was stored for 30 d, the soil pesticide residue degradation test was performed. The djl-6 bacteria agent with earthworm manure as a carrier degraded 94.30% of carbendazim at 5 mg/kg, and the D-2 bacteria with earthworm manure as a carrier degraded 81.87% of carbendazim at 10 mg/kg. The results of this study indicated that the solid pesticide residue degrading bacterial agent using earthworm manure as a carrier has a high number of live bacteria and a long shelf life, and has a good application prospect.