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多环芳烃长期污染土壤的微生物强化修复初步研究
引用本文:邹德勋,骆永明,滕应,平立凤,刘五星,李振高.多环芳烃长期污染土壤的微生物强化修复初步研究[J].土壤,2006,38(5):652-656.
作者姓名:邹德勋  骆永明  滕应  平立凤  刘五星  李振高
作者单位:1. 中国科学院南京土壤研究所土壤与环境生物修复研究中心,南京,210008;土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京,210008;东北农业大学资源与环境学院,哈尔滨,150030
2. 中国科学院南京土壤研究所土壤与环境生物修复研究中心,南京,210008;土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京,210008
基金项目:国家自然科学基金;国家高技术研究发展计划(863计划);国家重点基础研究发展计划(973计划);中国科学院知识创新工程项目
摘    要:本研究通过室内模拟试验,以急性毒性较强的菲(Phe)和遗传毒性较强的苯并a]芘(Ba]P)为代表性多环芳烃(PAHs)污染物,以不同C源、通气状况和水分条件为调控因子,对PAHs长期污染土壤的土著微生物强化修复进行初步研究。结果表明,搅动处理使污染土壤中Phe和Ba]P的降解率分别达59.44%和26.14%,而淹水处理使两者降解率分别达46.48%和13.27%。添加C源(淀粉和葡萄糖)处理提高了土壤中PAHs的降解率,且随着C源的施用量而增加。同时也发现污染土壤中PAHs降解菌和微生物总量呈正相关,并随着PAHs降解菌数量的增加,土壤中PAHs降解率也随之提高。可见,土壤中PAHs降解速率主要决定于PAHs的降解菌数量。

关 键 词:多环芳烃  污染土壤  微生物降解  PAHs降解菌

Enhanced Microbial Remediation of Long-term Polycyclic Aromatic Hydrocarbons (PAHs) Polluted Soils
ZOU De-xun,LUO Yong-ming,TENG Ying,PING Li-feng,LIU Wu-xing and LI Zhen-gao.Enhanced Microbial Remediation of Long-term Polycyclic Aromatic Hydrocarbons (PAHs) Polluted Soils[J].Soils,2006,38(5):652-656.
Authors:ZOU De-xun  LUO Yong-ming  TENG Ying  PING Li-feng  LIU Wu-xing and LI Zhen-gao
Institution:Soil and Environmental Bioremediation Research Centre, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Soil and Sustainable Agriculture(Institute of Soil Science, Chinese Academy of Sciences), Nanjing 210008, China;College of resources and environmental Sciences, Northeast Agricultural University, Haerbin 150030, China,Soil and Environmental Bioremediation Research Centre, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Soil and Sustainable Agriculture(Institute of Soil Science, Chinese Academy of Sciences), Nanjing 210008, China,Soil and Environmental Bioremediation Research Centre, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Soil and Sustainable Agriculture(Institute of Soil Science, Chinese Academy of Sciences), Nanjing 210008, China,Soil and Environmental Bioremediation Research Centre, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Soil and Sustainable Agriculture(Institute of Soil Science, Chinese Academy of Sciences), Nanjing 210008, China,Soil and Environmental Bioremediation Research Centre, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Soil and Sustainable Agriculture(Institute of Soil Science, Chinese Academy of Sciences), Nanjing 210008, China and Soil and Environmental Bioremediation Research Centre, Institute of Soil Science, Chinese Academy of Sciences, Nanjing 210008, China;State Key Laboratory of Soil and Sustainable Agriculture(Institute of Soil Science, Chinese Academy of Sciences), Nanjing 210008, China
Abstract:An incubation experiment was carried out with farmland soils long-term polluted by PAHs under different carbon sources, aeration condition, and soil moisture contents. The results showed that aeration treatment enhanced degradation rates of Phe and Ba]P in soils to 59.44% and 26.14%, while submerging treatment enhanced them to 46.48% and 13.27%, respectively. Glucose and starch carbon sources were beneficial to the growth of soil microorganisms, thus accelerated the degradation of PAHs in polluted soils. Soil PAHs degradation rate was not only correlated with carbon sources, but also closely correlated with their application contents. The results also indicated soil PAHs-degrading microorganisms were positively correlated with total microorganism numbers in long-term PAHs polluted soils.
Keywords:Polycyclic aromatic hydrocarbons (PAHs)  Polluted soils  Microbial degradation  PAHs-degrading bacteria
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