首页 | 本学科首页   官方微博 | 高级检索  
     检索      

病死动物源氨基酸研制的生物有机肥促生效果研究
引用本文:白国新,刘珊珊,郑 宇,邵 铖,王东升,李 荣,沈其荣.病死动物源氨基酸研制的生物有机肥促生效果研究[J].土壤,2018,50(2):270-276.
作者姓名:白国新  刘珊珊  郑 宇  邵 铖  王东升  李 荣  沈其荣
作者单位:国家有机类肥料工程技术研究中心 农业部长江中下游植物营养与肥料重点实验室 江苏省固体有机废弃物资源化高技术研究重点实验室 江苏省有机固体废弃物资源化协同创新中心 南京农业大学资源与环境科学学院,国家有机类肥料工程技术研究中心 农业部长江中下游植物营养与肥料重点实验室 江苏省固体有机废弃物资源化高技术研究重点实验室 江苏省有机固体废弃物资源化协同创新中心 南京农业大学资源与环境科学学院,国家有机类肥料工程技术研究中心 农业部长江中下游植物营养与肥料重点实验室 江苏省固体有机废弃物资源化高技术研究重点实验室 江苏省有机固体废弃物资源化协同创新中心 南京农业大学资源与环境科学学院,国家有机类肥料工程技术研究中心 农业部长江中下游植物营养与肥料重点实验室 江苏省固体有机废弃物资源化高技术研究重点实验室 江苏省有机固体废弃物资源化协同创新中心 南京农业大学资源与环境科学学院,国家有机类肥料工程技术研究中心 农业部长江中下游植物营养与肥料重点实验室 江苏省固体有机废弃物资源化高技术研究重点实验室 江苏省有机固体废弃物资源化协同创新中心 南京农业大学资源与环境科学学院,国家有机类肥料工程技术研究中心 农业部长江中下游植物营养与肥料重点实验室 江苏省固体有机废弃物资源化高技术研究重点实验室 江苏省有机固体废弃物资源化协同创新中心 南京农业大学资源与环境科学学院,国家有机类肥料工程技术研究中心 农业部长江中下游植物营养与肥料重点实验室 江苏省固体有机废弃物资源化高技术研究重点实验室 江苏省有机固体废弃物资源化协同创新中心 南京农业大学资源与环境科学学院;南京市蔬菜科学研究所
基金项目:国家自然科学基金项目(面上项目,重点项目,重大项目)
摘    要:废弃动物尸体酸解为氨基酸能够有效解决农业生产中动物尸体带来的环境污染问题,本研究利用此类病死动物资源水解的的氨基酸研制生物有机肥,以期在研制出低成本高品质生物有机肥的同时推进新型氨基酸的利用。本文首先比较了根际功能菌Bacillus amyloliquefaciens SQR9在添加不同浓度酸解氨基酸液有机肥中的固体发酵效果及不同浓度酸解氨基酸水解液发酵的生物有机肥盆栽促生效果,进而确立了生物有机肥的氨基酸添加浓度。再进一步通过黄瓜、辣椒和玉米盆栽试验,评估了所研制新型生物有机肥的促生效果。研究结果表明:酸解氨基酸水解液添加量为20%时,所研制生物有机肥中功能菌数量最高,达到2.25×10~8CFU/g,是未添加氨基酸对照的3倍。黄瓜盆栽促生试验表明,氨基酸添加量为20%所发酵的生物有机肥促生效果优于其他处理和对照,株高、茎粗、叶绿素、鲜重和干重值均为最高,根围土壤中功能菌的数量达2.51×10~5CFU/g。不同作物盆栽试验结果表明,添加氨基酸研制的含菌株SQR9生物有机肥处理的促生效果优于添加化肥研制的含菌株SQR9生物有机肥、有机肥直接接种菌株SQR9研制的生物有机肥、普通有机肥和添加氨基酸的普通有机肥处理,同时施用含SQR9菌株的肥料促生效果优于施用不含功能菌的相同配方制造的有机肥处理,表明,利用菌株SQR9研制的生物有机肥具有良好的促生效果,相比于化肥,添加氨基酸固体发酵功能菌后更加有益于功能菌促生功能的发挥。在各种作物根围土壤中的微生物涂布试验中,添加氨基酸研制的含菌株SQR9生物有机肥处理的功能微生物数量显著高于其他处理。综上,添加20%的氨基酸水解液能够有效促进功能微生物在有机肥中的繁殖,固体发酵形成的生物有机肥具有优异的促生效果。

关 键 词:解淀粉芽孢杆菌  SQR9  氨基酸水解液  废弃动物尸体  生物有机肥  促生
收稿时间:2017/2/23 0:00:00
修稿时间:2017/5/3 0:00:00

Plant Growth Promotion Effect of Novel Bio-organic Fertilizers Produced by Solid-state Fermentation with Amino Acids Hydrolyzed from Animal Carcasses as Additive
BAI Guoxin,LIU Shanshan,ZHENG Yu,SHAO Cheng,WANG Dongsheng,LI Rong and SHEN Qirong.Plant Growth Promotion Effect of Novel Bio-organic Fertilizers Produced by Solid-state Fermentation with Amino Acids Hydrolyzed from Animal Carcasses as Additive[J].Soils,2018,50(2):270-276.
Authors:BAI Guoxin  LIU Shanshan  ZHENG Yu  SHAO Cheng  WANG Dongsheng  LI Rong and SHEN Qirong
Institution:National Engineering Research Center for Organic-based Fertilizers,Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River,Ministry of Agriculture,Jiangsu Key Laboratory of Solid Organic Waste Utilization,Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization,Nanjing Agricultural University,National Engineering Research Center for Organic-based Fertilizers,Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River,Ministry of Agriculture,Jiangsu Key Laboratory of Solid Organic Waste Utilization,Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization,Nanjing Agricultural University,National Engineering Research Center for Organic-based Fertilizers,Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River,Ministry of Agriculture,Jiangsu Key Laboratory of Solid Organic Waste Utilization,Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization,Nanjing Agricultural University,National Engineering Research Center for Organic-based Fertilizers,Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River,Ministry of Agriculture,Jiangsu Key Laboratory of Solid Organic Waste Utilization,Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization,Nanjing Agricultural University,National Engineering Research Center for Organic-based Fertilizers,Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River,Ministry of Agriculture,Jiangsu Key Laboratory of Solid Organic Waste Utilization,Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization,Nanjing Agricultural University,National Engineering Research Center for Organic-based Fertilizers,Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River,Ministry of Agriculture,Jiangsu Key Laboratory of Solid Organic Waste Utilization,Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization,Nanjing Agricultural University,National Engineering Research Center for Organic-based Fertilizers,Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River,Ministry of Agriculture,Jiangsu Key Laboratory of Solid Organic Waste Utilization,Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization,Nanjing Agricultural University;China;Nanjing Institute of Vegetable Science;China
Abstract:Animal carcasses hydrolyzed by sulfuric acid solution to create compound liquid amino acids is an effectively strategy to protect the environment. However, how to efficiently utilize these liquid amino acids does cause public concern. In this study, compound liquid amino acids from animal carcasses were utilized as additives into matured composts to create novel bio-organic fertilizer (BIO) containing plant growth-promoting rhizobacteria (PGPR) Bacillus amyloliquefaciens SQR9, after that the plant growth promotion effects of novel bio-organic fertilizers were investigated. Results showed that the optimal amendment concentration of liquid amino acids was 20%, which resulted in a maximum biomass of functional strains with the number of 2.25×108 CFU/g (dry weight, the same follow) that was 3 times than the control without adding liquid amino acids. Pot experiment results showed that cucumber plant growth promotion effects of the novel bio-organic fertilizer were superior to the other treatments amended with different concentrations of liquid amino acids and the control. The number of strain SQR9 in rhizosphere soil reached 2.5 × 105 CFU/g soil. Results from pot experiments of different crops showed the novel produced BIO resulted in greater plant growth compared to the treatment amended with BIO produced by adding chemical fertilizer as additive, the treatment amended with BIO produced by adding none additive, the treatment amended with organic fertilizer and the treatment amended with organic fertilizer amended with liquid amino acids. Meanwhile, the treatments amended with the fertilizers with strain SQR9 showed better plant growth than the others, indicating that strain SQR9 exhibited plant growth promotion effect. Moreover, compared to the treatment amended with BIO produced by adding chemical fertilizer as additive, the treatment amended with novel BIO is more beneficial for the function enhancement of the strain SQR9. In the rhizosphere of different crops, significant higher number of functional microbes were observed in the treatment amended with novel BIO. To sum up, adding 20% of liquid amino acids effectively enhanced the growth of functional microbes in the organic fertilizer, and the produced novel BIO showed outstanding plant growth promotion effect.
Keywords:Bacillus amyloliquefaciens SQR9  compound liquid amino acids  animal carcasses  bio-organic fertilizer  plant growth promotion effect
本文献已被 CNKI 等数据库收录!
点击此处可从《土壤》浏览原始摘要信息
点击此处可从《土壤》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号