| LiCl沉淀法提取富含荚膜的细菌基因组DNA |
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| 引用本文： | 侯卫国,连宾.LiCl沉淀法提取富含荚膜的细菌基因组DNA[J].土壤,2006,38(6):774-777. |
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| 作者姓名： | 侯卫国 连宾 |
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| 作者单位： | 南京师范大学生命科学学院微生物工程重点实验室,南京,210097 |
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| 基金项目： | 江苏省普通高校高新技术产业发展项目;江苏省博士后科学基金 |
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| 摘 要： | 结合提取基因组DNA的CTAB法(hexadecyltrimethylammoniumbromide,十六烷基三甲基溴化铵)和用于提取海藻基因组DNA的LiCl法,设计了针对产生较多荚膜多糖细菌的DNA提取方法—LiCl沉淀法。通过电泳、分光光度计及16SrRNA基因扩增检测,证明LiCl沉淀法可以有效地提取产荚膜细菌基因组DNA,其片断大小约为23kb,不需进一步纯化即可用于后续分子生物学实验。
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| 关 键 词： | 夹膜多糖 细菌 基因组DNA提取 LiCl沉淀法 |
Genomic DNA Extraction from Bacteria Rich in Capsular Polysaccharides by LiCl Precipitating Method |
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| HOU Wei-guo and LIAN Bin.Genomic DNA Extraction from Bacteria Rich in Capsular Polysaccharides by LiCl Precipitating Method[J].Soils,2006,38(6):774-777. |
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| Authors: | HOU Wei-guo and LIAN Bin |
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| Institution: | Key Laboratory of Microbial Engineering, College of Life Sciences, Nanjing Normal University, Nanjing 210097, China |
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| Abstract: | Bacteria rich in capsule are widely applied in many fields and it is important to investigate their gene resources. But capsules, especially capsule polysaccharides, will disturb DNA extracting largely, thus, LiCl precipitating method was introduced in this paper as a novel method and was examined by electrophoresis, spectrophotometer and amplification of 16S rRNA gene. Results showed that it could extract genomic DNA about 23 kb effectively in molecular weight from bacteria rich in capsule polysaccharides, and doesn''t need purification for following serial molecular biological experiments. |
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| Keywords: | Capsular polysaccharides Bacteria Genomic DNA LiCl precipitating method |
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