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高耐铝红酵母RS1耐铝特性初步研究
引用本文:王 超,赵学强,沈仁芳.高耐铝红酵母RS1耐铝特性初步研究[J].土壤,2013,45(3):501-505.
作者姓名:王 超  赵学强  沈仁芳
作者单位:1. 土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京210008;中国科学院大学,北京100049
2. 土壤与农业可持续发展国家重点实验室(中国科学院南京土壤研究所),南京,210008
基金项目:国家自然科学基金项目(41025005;40871144;31000933)资助
摘    要:红酵母RS1是从酸性土壤中筛选出的一株高耐铝微生物,本文对其耐铝特性进行了初步研究.结果发现,RS1可以在pH 2.5的培养基中生长,50 ~ 200 mmol/L A13+处理对RS1产生了毒害作用,其毒害并未对细胞表面形态产生影响.RS1对Al2(SO4)3的忍耐能力高于AlCl3,这种高的忍耐能力是遗传固有的,并不被诱导产生.此外,RS1可以增加体内蛋白含量来适应高的铝毒,其中蛋白型巯基可能在耐铝中表现重要作用.

关 键 词:红酵母RS1  铝毒  pH  蛋白质  巯基

A Study on High Aluminum-tolerant Characteristic of Rhodotorula sp. RS1
WANG Chao,ZHAO Xue-qiang,SHEN Ren-fang.A Study on High Aluminum-tolerant Characteristic of Rhodotorula sp. RS1[J].Soils,2013,45(3):501-505.
Authors:WANG Chao  ZHAO Xue-qiang  SHEN Ren-fang
Institution:State Key Laboratory of Soil and Sustainable Agriculture (Institute of Soil Science, Chinese Academy of Sciences)
Abstract:Rhodotorula sp. RS1 isolated from acidic oil-tea fields is a high Al-tolerant microorganism. In this study, its Al-tolerant characteristic was studied. Results showed that the growth of RS1 colony was inhibited under the treatment of 50-200 mmol/L Al3+; however, Al toxicity did not obviously influence the cell morphology. RS1 showed higher tolerant ability for Al2(SO4)3 than AlCl3, and this high Al-tolerant ability was not induced by Al3+. Under the treatment of highly Al toxicity, RS1 could increase protein content, and protein thiols may play an important role in high tolerance of RS1 to Al.
Keywords:Rhodotorula sp  RS1  Al toxicity  pH  Protein  Thiols
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