Lentil (Lens culinaris L.) growth promoting rhizobacteria and their effect on nodulation in coinoculation with rhizobia |
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Authors: | Maurine Sepúlveda-Caamaño Marisol Vargas Ernesto Moya-Elizondo Pía Oyarzúa Jorge Campos |
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Institution: | 1. Producción Vegetal, Facultad de Agronomia, Universidad de Concepcion, Chillan, Chile;2. Producción Animal, Facultad Agronomia, Universidad de Concepción, Chillan, Chile |
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Abstract: | Lentil is cultivated in Chilean Mediterranean drylands, in areas with soils that are nutrient depleted and eroded. Inoculation of lentil with rhizobia in co-inoculation with growth promoting rhizobacteria would allow higher biomass and an opportunity for early nodulation and increased nitrogen fixation. The objective of this research was to select rhizosferic bacteria (PGPR) from lentils and to evaluate their effect on lentil nodulation in co-inoculation with rhizobia. Sixty three lentil rhizobacteria isolates where obtained from nine soils in the mediterranean area. These were fingerprinted through BOX-PCR reducing the number to 57 distinct strains. The strains were evaluated for ACCdeaminase activity, IAA production and compatibility with rhizobia. Seventeen strains showed ACC-deaminase activity, all of them synthesized IAA and 38 were compatible with the rhizobia. Ten selected strains were identified as Pseudomonas spp. through 16S rRNA sequencing. The strains were inoculated in lentil seedlings growing on seed germination pouches, to evaluate nodule formation. The strain LY50a increased early nodulation in 85% in comparison to the control inoculated with rhizobia (AG-84) only. In conclusion, bacteria from the rhizosphere from Mediterranean soils of Chile can be used as nodulation promoters in lentils. |
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Keywords: | PGPR Pseudomonas Rhizobium leguminosarum bv vicieae indole acetic acid ACCdeaminase |
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