首页 | 本学科首页   官方微博 | 高级检索  
     检索      

秸秆与菇渣堆肥用于双孢蘑菇栽培的理化性状和细菌群落分析
引用本文:秦改娟,郭亚萍,张国庆,陈青君.秸秆与菇渣堆肥用于双孢蘑菇栽培的理化性状和细菌群落分析[J].农业工程学报,2016,32(Z2):285-291.
作者姓名:秦改娟  郭亚萍  张国庆  陈青君
作者单位:1. 北京农学院植物科学技术学院农业应用新技术北京市重点实验室,北京,102206;2. 北京农学院生物科学与工程学院农业部都市农业 北方 重点实验室,北京,102206
基金项目:十二五国家科技支撑计划项目"农牧业废弃物资源菌业循环子课题" (2012BAD14B09);现代农业产业技术体系北京市食用菌创新团队建设双孢菇工厂化关键技术研究(PXM2016-014207-000036);北京市科技新星项目(xx2015B025)。
摘    要:该研究以双孢蘑菇(Agaricus bisporus)传统麦草配方作为对照,分别用玉米秸秆和杏鲍菇菇渣替换部分麦草设计2个新配方,通过理化性状、细菌群落动态及产量分析,评价玉米秸秆或杏鲍菇菇渣用于工厂化双孢菇生产的可行性。分别在建堆、一次发酵结束、二次发酵结束3个时期采集堆肥样品,提取总DNA,以大肠杆菌(Escherichia coli)16S r DNA V3区通用引物,进行PCR‐DGGE扩增和序列分析,同时分析培养料理化性状,并测定双孢蘑菇产量。研究结果:玉米秸秆配方基质颗粒比较粗大,含氮量较低,二次发酵结束时与麦草配方具有较多的共有优势菌群,两者的细菌多样性指数变化规律也基本一致。菇渣配方基质颗粒比较细碎,含氮量较高,其堆肥3个时期的优势细菌种类、多样性指数变化趋势与麦草配方明显不同。传统麦草配方具有良好的理化性状和稳定的细菌群落,玉米秸秆、杏鲍菇菇渣代替部分麦草的配方仍需进一步优化。

关 键 词:堆肥  细菌  秸秆  菇渣  理化性状  PCR-DGGE  双孢蘑菇
收稿时间:2016/7/21 0:00:00
修稿时间:2016/9/13 0:00:00

Physical-chemical properties and bacterial community analysis of different composts made by straws or oyster mushroom residues for commerical mushroom Agaricus bisporus
Qin Gaijuan,Guo Yaping,Zhang Guoqing and Chen Qingjun.Physical-chemical properties and bacterial community analysis of different composts made by straws or oyster mushroom residues for commerical mushroom Agaricus bisporus[J].Transactions of the Chinese Society of Agricultural Engineering,2016,32(Z2):285-291.
Authors:Qin Gaijuan  Guo Yaping  Zhang Guoqing and Chen Qingjun
Institution:1. College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206, China,1. College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206, China,2. College of Biological Sciences and Engineering, Key Laboratory of Urban Agriculture of Ministry of Agriculture, Beijing University of Agriculture, Beijing 102206, China and 1. College of Plant Science and Technology, Beijing Key Laboratory for Agricultural Application and New Technique, Beijing University of Agriculture, Beijing 102206, China
Abstract:Abstract: Using the traditional wheat straw formula as control, two test compost formulas were designed using corn straws or oyster mushroom (Pleurotus eryngii) residues replacing part of the wheat straw, respectively. At the beginning of composting, the end of phase I, and phase II, compost samples were collected and total DNA were further obtained. Bacterial communities of each sample were analysed using Polymerase Chain Reaction-Denaturing gradient gel electrophoresis (PCR-DGGE) method based on 16S rDNA using the universal primers from Escherichia coli. The physical-chemical properties of the samples and the yield of the three formula were also measured and analysed. The results showed that the corn stalks formula shared higher particle size and lower nitrogen content than those of the wheat straw formula. At the end of phase II, the corn stalks formula demonstrated considerably high similarity of dominant bacteria with the wheat straw formula. The two formulas also shared similar bacterial diversity variation trends. The oyster mushroom residues formula shared tiny particle size, high nitrogen content. The dominant bacteria species and bacterial diversity variation were highly different from those of the wheat straw formula.
Keywords:composting  bacterial  straws  oyster mushroom residues  physical-chemical properties  PCR-DGGE  Agaricus bisporus
本文献已被 CNKI 万方数据 等数据库收录!
点击此处可从《农业工程学报》浏览原始摘要信息
点击此处可从《农业工程学报》下载免费的PDF全文
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司  京ICP备09084417号