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接种木质纤维素分解复合菌系对堆肥发酵进程的影响
引用本文:王伟东,王小芬,王彦杰,荆瑞勇,崔宗均.接种木质纤维素分解复合菌系对堆肥发酵进程的影响[J].农业工程学报,2008,24(7):193-198.
作者姓名:王伟东  王小芬  王彦杰  荆瑞勇  崔宗均
作者单位:1. 黑龙江八一农垦大学植物科技学院,大庆,163319;黑龙江八一农垦大学生命科技学院,大庆,163319
2. 中国农业大学农学与生物技术学院,北京,100094
3. 黑龙江八一农垦大学生命科技学院,大庆,163319
基金项目:国家"十一五"科技支撑项目 , 黑龙江省"十一五',重大科技攻关项目 , 黑龙江省普通高等学校新世纪优秀人才培养计划 , 黑龙江省农垦总局科技攻关项目 , 黑龙江八一农垦大学博士基金
摘    要:以具有高效木质纤维素分解能力的复合菌系作为接菌剂,接种到以牛粪、鸡粪和麦秸为材料的堆肥化过程中,测定了各发酵参数和物质成分的变化.利用变性梯度凝胶电泳(DGGE)和末端限制性片断多态性(T-RFLP)分析方法,研究了堆肥发酵过程中微生物群落的动态.结果表明,接菌对堆肥化过程中温度、水分和pH值的影响不大.经63 d发酵之后,接菌处理的半纤维素、纤维素和木质素的减重率比不接菌处理分别高4.3%、3.0%和3.4%.接菌处理的各个发酵阶段C/N比也明显低于不接菌处理.DGGE和T-RFLP的结果显示,接菌后堆肥发酵初期复合系中的 Ureibacillus thermosphaericu、Pseudoxanthomonas taiwanensis、Tepidiphilus margaritifer、Rhizobiaceae str.M100、C thermobutyricum 和Bacillus thermoamylovorans 菌株大量定殖于堆肥体系中,DGGE图谱中可见接种处理堆肥体系中的条带数少于不接菌处理,而同一水平线的条带亮度明显高于不接菌处理.DGGE和T-RFLP的结果都表明接菌处理的微生物多样性少于不接菌处理.可见接种微生物在堆肥体系中占据优势,抑制了部分杂菌生长,促进了发酵进程.

关 键 词:木质纤维素  复合系  接菌剂  堆肥  微生物群落
收稿时间:2007/9/27 0:00:00
修稿时间:2008/1/28 0:00:00

Effect of microbial inoculum with high lignocellulose degradation ability on composting process
Wang Weidong,Wang Xiaofen,Wang Yanjie,Jing Ruiyong and Cui Zongjun.Effect of microbial inoculum with high lignocellulose degradation ability on composting process[J].Transactions of the Chinese Society of Agricultural Engineering,2008,24(7):193-198.
Authors:Wang Weidong  Wang Xiaofen  Wang Yanjie  Jing Ruiyong and Cui Zongjun
Institution:1. College of Plant Science and Technology, Heilongjang August First Land Reclamation University, Daqing 163319, China; 2. College of Biological Science and Technology, Heilongjang August First Land Reclamation University, Daqing 163319, China,College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China,College of Biological Science and Technology, Heilongjang August First Land Reclamation University, Daqing 163319, China,College of Biological Science and Technology, Heilongjang August First Land Reclamation University, Daqing 163319, China and College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China
Abstract:Microbial inoculum that is made up of composite bacterial system capable of lignocellulose degradation was applied in composting system composed of cattle manure, chicken manure, and wheat straw for a period of 63 days. Fermentation parameters were analyzed and microbial community succession was detected with methods of denaturing gradient gel electrophoresis(DGGE) and terminal restriction fragment length polymorphism(T-RFLP) during composting process. The results show that the differences of temperature, moisture, and pH value between inoculated treatment and non-inoculated treatment were insignificant during composting process. Degradation ratios of hemicellulose, cellulose and lignin under inoculated treatment were 4.3%, 3.0% and 3.4% higher than those under non-inoculated treatment, respectively. The C/N ratio of compost material at different fermentation phases under inoculated treatment was lower than that under non-inoculated treatment. Ureibacillus thermosphaericu, Pseudoxanthomonas taiwanensis, Tepidiphilus margaritifer, Rhizobiaceae str. M100, C. thermobutyricum and Bacillus thermoamylovorans, which are the ingredients of composite bacterial system, were detected with methods of DGGE at later phase during composting process. Moreover, band numbers of DGGE figure under inoculated treatment were fewer than those under non-inoculated treatment, but band density of DGGE figure under inoculated treatment was denser than that under inoculated treatment. Microbial diversity under inoculated treatment was fewer than that under non-inoculated treatment proved by DGGE and T-RFLP. Composting process was improved by microbial inoculum.
Keywords:lignocellulose  composite bacterial system  inoculum  compost  microbial community
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