原料连续添加和酸酶循环利用法(CACU法)降解植物纤维素新技术

以乙酸、草酸、盐酸、硫酸等单酸、二酸、三酸混和及纤维素酶降解植物纤维素的正交试验得出的最佳工艺条件为基础,进一步研究出一种原料连续添加和酸酶循环利用法降解植物纤维素新技术(CACU法)。该技术的整个过程只需要12步操作,5或6套设备,包括3～4套反应釜和2套贮罐。在常压、温度100℃、反应15h的条件下,以CH₃COOH,HCl和纤维素酶为反应剂,按照该工艺技术能使纤维素转化成葡萄糖的转化率达95.34%,反应终液中的葡萄糖浓度达3.21%。与常规技术相比,该新技术只需25%的醋酸和50%的盐酸量,可以较大幅度地降低纤维素转化成葡萄糖的成本。因此,该技术具有条件要求低、工艺简单、成本低和周期短等特点,是一种值得进一步开发和推广的技术。

A Novel Approach to Degrading Plant Cellulose:Continual Adding Materials and Cycling Utilization of Acids and Cellulase

A novel approach to degrading plant cellulose--Continual Adding Materials and Cycling Utilization of Acids and Cellulase(CACU) is developed on the basis of the optimum results of degrading cellulose with single acid, double or multiple acids and cellulase-lyses for short time by orthogonal experiments. The schematic flow diagram for continual adding materials and cycling utilization of acids and enzymes for the production of glucose from cellulose was designed, drawn and described. The experimental results show that the CACU method is an effective way of degrading cellulose, which possesses the properties of common conditions, simple process, lower cost and a short period. The whole system consists of five or six sets of equipment, including three sets of reaction equipment and two sets of plant containers. There are totally twelve steps of operation in the whole process. The final transformation ratio of glucose to cellulose and the final concentration of glucose in the end-residue can increase up to 95.34% and 3.21%, respectively, with CH3COOH, HCl and cellulase at 100℃, 1 kg/cm2, and for 15 h by the CACU method. It consumes a quarter of acetic acid and half of HCl compared with the traditional way. The CACU method can decrease the cost of production of glucose from cellulose greatly. Thus, the CACU method is worthy to be developed and spread because of its excellent properties.