传统风干牦牛肉加工中肌原纤维蛋白氧化与体外消化性变化

为了评价传统风干牦牛肉在加工过程中肌原纤维蛋白消化率的变化,并探讨蛋白质氧化影响其消化性的潜在机制。在牦牛肉自然风干加工过程中(40 d)采集样本,测定肌原纤维蛋白羰基、巯基、二硫键、二聚酪氨酸、粒径分布、体外消化率及脂质氧化等指标,并分析脂质氧化和蛋白质氧化对蛋白质消化性的影响。结果显示,脂质初级氧化产物(Primary Oxidation Value,POV)、硫代巴比妥酸反应物(Thiobarbituric Acid Reaction Substrates,TBARS)和蛋白质羰基化合物随加工时间的延长而显著增加(P<0.05),并且相互间存在极显著相关性(P<0.01)。此外,二硫键和二聚酪氨酸含量显著增加(P<0.05),这是蛋白质交联的主要形式。蛋白质被胃蛋白酶和胰蛋白酶总的水解率在整个加工过程中降低了19.58%(P<0.05),蛋白质的水解率与羰基、二硫键、二聚酪氨酸和蛋白质粒径呈现极显著负相关性(P<0.01)。综上所述,在风干牛肉加工期间,脂质氧化产物促进了蛋白质氧化,蛋白质的羰基化和交联导致蛋白酶对蛋白质的水解率降低。研究结果对了解风干牦牛肉传统加工方式和进一步改进加工工艺具有积极的促进作用。

Changes of myofibrillar protein oxidation and in vitro digestion in yak meat during air drying

Air-dried yak meat can be produced by a traditional procedure,where the raw yak meat is divided into strips,then naturally air-drying(about 40 days)under a low temperature condition.This ancient processing is still popular in China at present,particularly on the high altitudes areas(generally over 3000 m),probably due to the influence of traditional eating habits and inheritance of culture.High-protein yak meat can be used to enhance the resistance of cold climates in these areas.However,the protein digestibility of air-dried yak meat has not been evaluated during traditional processing.Consequently,the objective of this study was to evaluate the changes in digestibility of myofibrillar protein during the processing of traditional air-dried yak meat,and further to investigate the underlying effect of protein oxidation on protein digestion.Taking the healthy 3 to 4-year-old male yaks as subject,the semimembranosus was selected to remove the fascia and fat on the surface,and then cut into a cross-section of 2×2 cm2 strip in Gannan prefecture of Gansu province.The meat was hung on the wire,and kept the distance of 1 to 2 cm between two adjacent pieces of meat in a dark ventilated naturally place in Xiahe County,Gannan Prefecture,Gansu Province.The temperature was set as-10 to-15℃,and the relative humidity was 50%to 75%.During air drying,changes were monitored in lipid oxidation,protein oxidation,protein particle distribution,and in vitro digestibility,in order to explore the effects of lipid and protein oxidation on protein digestibility.Samples were collected to analyze during natural air-drying for 40 days.A model was introduced to simulate the digestion of proteins in the stomach and duodenum,specifically to investigate the nutritional change of the air-dried yak meat in the processing.The results showed that the lipid Primary Oxidation Value(POV),Thiobarbituric Acid Reaction Substrate(TBARS)and protein carbonyl compounds increased dramatically with the extension of processing time(P<0.05),and there was a significant correlation between them(P<0.01).In addition,the content of disulfide bonds and dimeric tyrosine increased significantly(P<0.05),which is the main form of protein cross-linking.The digestibility of protein decreased by 19.58%during the whole process(P<0.05).The hydrolysis rate of protein by protease showed a significant correlation with the carbonyl group,disulfide bond,dimeric tyrosine,and protein particle size(P<0.01).Lipid oxidize products can promote protein oxidation,while the carbonylation and cross-linking of proteins can cause a decrease in protein hydrolysis rate by proteases.This study has a positive role to understand the traditional processing of air-dried yak meat,and further to improve processing technology and quality of yak meat.